Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples.

IF 1.5 4区工程技术 Q3 MICROSCOPY

Microscopy Pub Date : 2019-12-03 DOI:10.1093/jmicro/dfz031

Tomohiro Haruta, Keiko Hasumi, Y. Ikeda, Y. Konyuba, T. Fukuda, H. Nishioka

引用次数: 4

Abstract

In this study, we investigated use of local surface plasmon resonance (LSPR) of metal nanoparticles (NPs) as a correlative light and electron microscopy (CLEM) tag for biological samples. Gold NPs in ultra-thin sections for TEM revealed that LSPR could be observed by optical microscopy at sizes of 20 nm or larger. Gold NPs at sizes less than 20 nm could be observed using the gold enhancement method. Therefore, this CLEM tag could be applied to immunoelectron microscopy using this gold enhancement method.

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金纳米粒子的局部表面等离子体共振作为生物样品的相关光学和电子显微镜(CLEM)标签。

在本研究中，我们研究了金属纳米颗粒（NP）的局部表面等离子体共振（LSPR）作为生物样品的相关光学和电子显微镜（CLEM）标签的使用。用于TEM的超薄切片中的金NP显示，可以通过光学显微镜在20nm或更大的尺寸下观察到LSPR。使用金增强方法可以观察到尺寸小于20nm的金NP。因此，这种CLEM标签可以应用于使用这种金增强方法的免疫电子显微镜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microscopy Physics and Astronomy-Instrumentation

CiteScore

3.30

自引率

11.10%

发文量

期刊介绍： Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.