Brine-Shrimp Lethality Bioassay of different extracts of the medicinal plant matricaria (chamomilla) flowers

Amal Ali, S. Ali, Suad Abd Elgadir Abdo EL-Rhman, M. Mohamed, T. Khider
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引用次数: 1

Abstract

The present study aimed to throw some light on phytochemical constituents of Matricaria (cammomile) flowers. The grinded flowers were extracted successively by petroleum ether (40-60°C), chloroform, 95% ethanol, and ethyl acetate; using soxhlet apparatus respectively. The extracts were concentrated under vacuum (rotatory evaporator).Phytochemical screening of matricaria flowers, were performed using standard methods, showed the presence of alkaloids, flavonoids, terpenoids, saponins, and anthraquinone. The 95% ethanolic crude extract was subjected to column chromatography and nine fractions were collected. Fractions 1-6 have the same Rf value, (using thin layer pre-coated silica gel (0.25cm). were combined and subjected to spectroscopic analysis; infrared spectra showed absorption at 1860, 1637 and 805 cm’ indicating the presence of c=o, c=c, and c-o groups respectively. Ultra violet absorption at 250-283nm assign for the presence of flavonoids, (lit. 250-280). Finally GC/MS spectra performed the presence flavonoids.Crude extracts (ethanol, methanol, petroleum ether, and chloroform) were investigated for antimicrobial activities against four strains of bacteria (Staphylococcus aureus, Escherichia coli, Bacillus sub tills, Pseudomonas aeruginosa, and two fungi: Asperigillus niger, and Candida albican) with disc diffusion method, the diameter of growth inhibition zone ranged 15-22mm.The distilled essential oil (hydro distillation) of matricaria flowers was subjected to GC/MS, a Number of aliphatic compounds were detected e.g. 2, 3 heptadione and 3-hexa none, 2, 5 dimethyl.
药用植物洋甘菊花不同提取物的盐水对虾致死性生物测定
本研究旨在为了解Matricaria(cammomile)花的植物化学成分提供一些依据。研磨后的花依次用石油醚(40-60°C)、氯仿、95%乙醇和乙酸乙酯提取;分别使用soxhlet装置。提取液在真空(旋转蒸发器)下浓缩。采用标准方法对基质花进行植物化学筛选,结果表明基质花中存在生物碱、黄酮、萜类、皂苷和蒽醌。对95%乙醇粗提取物进行柱色谱,并收集9个级分。级分1-6具有相同的Rf值(使用薄层预涂硅胶(0.25cm)),合并并进行光谱分析;红外光谱在1860、1637和805厘米处显示吸收,分别表明存在c=o、c=c和c-o基团。250-283nm处的紫外线吸收表明存在黄酮类化合物(插图250-280)。最后用GC/MS对黄酮类化合物的存在进行了分析。采用圆盘扩散法研究了粗提取物(乙醇、甲醇、石油醚和氯仿)对四种细菌(金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌、铜绿假单胞菌和两种真菌:黑曲霉和白色念珠菌)的抗菌活性,生长抑制区的直径在15-22mm之间。对基质花的蒸馏精油(加氢蒸馏)进行GC/MS,检测到一些脂肪族化合物,如2,3-庚二酮和3-己酮,2,5-二甲基。
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