Evaluation of reproducibility uncertainty in micropipette calibrations for non-nominal volumes through an interlaboratory study

IF 0.8 4区工程技术 Q4 CHEMISTRY, ANALYTICAL

Accreditation and Quality Assurance Pub Date : 2021-02-23 DOI:10.1007/s00769-020-01453-5

Katsuhiro Shirono, Hideyuki Tanaka

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Abstract

The nominal volume of a variable volume micropipette is the maximum volume of the range specified by the micropipette manufacturer. The purpose of the present study is to quantify the reproducibility standard uncertainty for non-nominal volume micropipette calibrations for variable volume micropipettes. An interlaboratory study was implemented for this purpose, using variable volume micropipettes with nominal volumes of 10?μL, 200?μL, and 1000?μL and setting the measurement volumes as 10?%, 50?%, and 100?% of the nominal volumes. In our previous paper (Accredit Qual Assur 19:377, 2014), we quantified the uncertainty due to reproducibility using only data obtained for nominal volume calibrations. From the results obtained, we found that the reproducibility uncertainties quantified for nominal volumes are too small to cover interlaboratory variations for non-nominal volume calibrations. For calibrations of 10?% and 50?% of the nominal volumes, the reproducibility uncertainties must be increased by the factors of 2.7 and 1.4, respectively, for micropipettes with nominal volumes of 10?μL to 1 000?μL, based on the results of this study.

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通过实验室间研究评估非标称体积微移管校准的再现性不确定度

可变体积微移液器的标称体积是微移液器制造商指定范围内的最大体积。本研究的目的是量化可变体积微移液器非标称体积微移液器校准的再现性标准不确定度。为此目的实施了一项实验室间研究，使用标称体积为10?μL, 200 ?μL, 1000?μL，并将测量音量设置为10?%, 50 ?%和100?%的标称体积。在我们之前的论文(Accredit qualal Assur 19:377, 2014)中，我们仅使用标称体积校准获得的数据量化了由于再现性引起的不确定性。从所获得的结果中，我们发现标称体积量化的再现性不确定度太小，无法覆盖非标称体积校准的实验室间变化。校准10?%和50?对于标称体积为10? ?的微移液器，其重复性不确定度必须分别增加2.7和1.4倍。μL到1 000?μL，基于本研究的结果。

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来源期刊

Accreditation and Quality Assurance 工程技术-分析化学

CiteScore

1.80

自引率

22.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Accreditation and Quality Assurance has established itself as the leading information and discussion forum for all aspects relevant to quality, transparency and reliability of measurement results in chemical and biological sciences. The journal serves the information needs of researchers, practitioners and decision makers dealing with quality assurance and quality management, including the development and application of metrological principles and concepts such as traceability or measurement uncertainty in the following fields: environment, nutrition, consumer protection, geology, metallurgy, pharmacy, forensics, clinical chemistry and laboratory medicine, and microbiology.