Zinc Chloride Supplemented during Ovarian Tissue Vitrification Improves In Vitro Follicle Development and Fertilization in Pigs

Q4 Multidisciplinary

Ohio Journal of Sciences Pub Date : 2021-10-04 DOI:10.18061/ojs.v121i2.7703

E. Hicks, Megan Martz, H. Arena, J. Rheubert, B. Whitaker

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引用次数: 0

Abstract

Ovarian tissue vitrification is a promising method to preserve follicles and gametes, but can be improved with mineral supplementation to the vitrification medium. The objective of this study was to determine the effects of supplementing 0.5 mg/mL zinc chloride to the media during cryopreservation of pig ovarian tissue. After thawing, the following criteria were evaluated: (1) follicular development and damage, (2) in vitro fertilization (IVF) characteristics, and (3) embryonic development. The number of damaged antral follicles (72.0 ± 3.8%) was less (p < 0.05) in ovarian tissue vitrified in media supplemented with zinc chloride compared to those not supplemented with zinc chloride (86.7 ± 4.1%). Oocytes obtained from the antral follicles on ovarian tissue vitrified in media supplemented with zinc chloride had less (p < 0.05) polyspermic penetration and higher (p < 0.05) male pronuclear formation during IVF than oocytes obtained from ovarian tissue not supplemented with zinc chloride. There were no statistical differences in embryonic development rates. Based on these results, supplementing zinc chloride during the vitrification protocol improves follicular development and subsequent IVF in pigs.

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卵巢组织玻璃化过程中添加氯化锌促进猪体外卵泡发育和受精

卵巢组织玻璃化是保存卵泡和配子的一种很有前途的方法，但可以通过在玻璃化培养基中补充矿物质来改善。本研究的目的是确定在猪卵巢组织冷冻保存过程中向培养基中补充0.5mg/mL氯化锌的效果。解冻后，评估以下标准：（1）卵泡发育和损伤，（2）体外受精（IVF）特征，以及（3）胚胎发育。与未补充氯化锌的卵母细胞（86.7±4.1%）相比，在添加氯化锌的培养基中玻璃化的卵巢组织中受损的窦状卵泡数量（72.0±3.8%）较少（p<0.05）与从未补充氯化锌的卵巢组织获得的卵母细胞相比，IVF过程中的原核形成。胚胎发育率没有统计学差异。基于这些结果，在玻璃化方案中补充氯化锌可以改善猪的卵泡发育和随后的试管婴儿。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ohio Journal of Sciences Multidisciplinary-Multidisciplinary

CiteScore

0.30

自引率

0.00%

发文量

期刊介绍： Published quarterly, plus the Annual Meeting Program Abstracts, The Ohio Journal of Science is the official publication of the Academy. The Journal publishes peer-reviewed, refereed papers contributing original knowledge to science, engineering, technology, education and their applications. The Journal is indexed and abstracted by many of the world"s leading indexing and abstracting services including State Academies of Science Abstracts which indexes the past 50 years of The Ohio Journal of Science.