Identification and pathogenicity of Nigrospora sphaerica and Curvularia penniseti associated with leaf spot disease of cowpea (Vigna unguiculata (L.) Walp.) in Nigeria

IF 1 Q3 PLANT SCIENCES

Archives of Phytopathology and Plant Protection Pub Date : 2023-03-16 DOI:10.1080/03235408.2023.2195035

I. Obisesan, P. Ojo

{"title":"Identification and pathogenicity of Nigrospora sphaerica and Curvularia penniseti associated with leaf spot disease of cowpea (Vigna unguiculata (L.) Walp.) in Nigeria","authors":"I. Obisesan, P. Ojo","doi":"10.1080/03235408.2023.2195035","DOIUrl":null,"url":null,"abstract":"Abstract Pathogenic fungi are one of the main causes of plant diseases due to their ability to colonise plants and rapidly spread disease. Cowpea is one of the most highly consumed grain legumes in Nigeria, but its yearly production is usually affected by fungi attacks. Disease presenting browning of the aerial parts of the cowpea plant was observed during the dry season between 2021 and 2022 in the country. This disease caused stunted growth in the affected plant stand. The fungi were isolated from the symptomatic aerial parts. The fungi were viewed under the microscope and identified by molecular and phylogenetic tools using the Internal Transcribed Spacer (ITS) gene region. The fungi isolated from the symptomatic aerial part of cowpea were identified as Nigrospora sphaerica and Culvularia penniseti. The pathogenicity of the isolated fungi was tested on detached leaves and stems and on intact cowpea seedlings. The test gave a positive result, which confirms the pathogenicity of the isolated fungi on the cowpea plant. This is the first report of N. sphaerica and C. penniseti infecting cowpea cultivated on farmland in Nigeria. Early detection of foreign fungi causing disease in the country’s cowpea crop will aid researchers in addressing the negative effects on crop yield.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Phytopathology and Plant Protection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/03235408.2023.2195035","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract Pathogenic fungi are one of the main causes of plant diseases due to their ability to colonise plants and rapidly spread disease. Cowpea is one of the most highly consumed grain legumes in Nigeria, but its yearly production is usually affected by fungi attacks. Disease presenting browning of the aerial parts of the cowpea plant was observed during the dry season between 2021 and 2022 in the country. This disease caused stunted growth in the affected plant stand. The fungi were isolated from the symptomatic aerial parts. The fungi were viewed under the microscope and identified by molecular and phylogenetic tools using the Internal Transcribed Spacer (ITS) gene region. The fungi isolated from the symptomatic aerial part of cowpea were identified as Nigrospora sphaerica and Culvularia penniseti. The pathogenicity of the isolated fungi was tested on detached leaves and stems and on intact cowpea seedlings. The test gave a positive result, which confirms the pathogenicity of the isolated fungi on the cowpea plant. This is the first report of N. sphaerica and C. penniseti infecting cowpea cultivated on farmland in Nigeria. Early detection of foreign fungi causing disease in the country’s cowpea crop will aid researchers in addressing the negative effects on crop yield.

查看原文本刊更多论文

与豇豆叶斑病相关的球形黑孢菌和弯孢菌的鉴定及致病性在尼日利亚

摘要病原真菌具有在植物中定植并迅速传播疾病的能力，是引起植物病害的主要原因之一。豇豆是尼日利亚消费量最高的谷物豆类之一，但其年产量通常受到真菌袭击的影响。在2021年至2022年的旱季，该国观察到豇豆植株地上部分出现病害褐变。这种疾病导致受影响的林分生长迟缓。真菌是从有症状的地上部分分离出来的。在显微镜下观察真菌，并使用内部转录间隔区（ITS）基因区通过分子和系统发育工具进行鉴定。从豇豆症状性地上部分分离到的真菌被鉴定为球形黑孢菌和狼尾草。分离的真菌在分离的叶和茎以及完整的豇豆幼苗上进行了致病性测试。试验结果为阳性，证实了分离真菌对豇豆植株的致病性。这是首次报道N.sphaerica和C.peniseti感染尼日利亚农田种植的豇豆。在该国豇豆作物中及早发现致病的外来真菌将有助于研究人员解决对作物产量的负面影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Archives of Phytopathology and Plant Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.20

自引率

0.00%

发文量

100

期刊介绍： Archives of Phytopathology and Plant Protection publishes original papers and reviews covering all scientific aspects of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology, herbal studies and applied nematology and entomology as well as strategies and tactics of protecting crop plants and stocks of crop products against diseases. The journal provides a permanent forum for discussion of questions relating to the influence of plant protection measures on soil, water and air quality and on the fauna and flora, as well as to their interdependence in ecosystems of cultivated and neighbouring areas.