Single shot quantitative phase gradient estimation using Wigner-Ville distribution in digital holographic microscopy

Abstract

In digital holographic microscopy, direct measurement of phase gradient is commonly required for investigations pertaining to cell morphology and feature detection. In this paper, we present the application of the Wigner-Ville distribution method for single shot quantitative phase gradient measurement in digital holographic microscopy. The method allows simultaneous extraction of phase derivatives along the horizontal and vertical dimensions to compute the phase gradient without the need of multiple images, filtering operations, or specialized experimental setups. Experimental results for imaging onion and cheek cells and standard calibration target, validates the potential of the proposed approach in digital holographic microscopy.