Research Article: A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish

IF 0.8 4区农林科学 Q3 FISHERIES

Iranian Journal of Fisheries Sciences Pub Date : 2020-11-10 DOI:10.22092/IJFS.2020.122798

M. Golshan, A. Hatef, H. Habibi, S. M. Alavi

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引用次数: 1

Abstract

Bisphenol A (BPA) is a synthetic compound widely used in types of consumer goods and medical tools. It has been shown that BPA acts as an endocrine disruptor chemical casuing negative impact on reproductive functions. The present study used an in vitro approach to assess estrogenic transcriptional activity of BPA as a rapid and sensitive method. The cultured pieces of hepatic tissue of goldfish (Carassius auratus) were exposed to 1, 5, 25 μg/L BPA and 1 µg/L E2 for comparison over 24 h. Compared to control, the mRNA transcript of erβ-I showed a significant increase in the hepatic tissues exposed to all doses of BPA and E2. The mRNA transcript of erβ-II was signifincantly increased in hepatic tissues exposed to 1 and 5 μg/L BPA and E2. The mRNA transcripts of vtg in the hepatic tissues treated with 5 μg/L BPA and E2 were also increased, significantly. Finally, cyp1a mRNA transcript showed a significant increase in the hepatic tissues exposed to 5 and 25 μg/L BPA and E2. These results show an estrogenic activity of BPA similar to E2, and suggest that in vitro approach can be used as an alternative to in vivo test to detect estrogenic effects of BPA.

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研究文章：一种快速评估双酚A在金鱼肝脏中雌激素转录活性的方法

双酚A（BPA）是一种合成化合物，广泛用于各类消费品和医疗工具。研究表明，BPA是一种内分泌干扰物，对生殖功能产生负面影响。本研究采用体外方法来评估BPA的雌激素转录活性，这是一种快速灵敏的方法。将金鱼（Carassius auratus）的肝组织培养片暴露于1、5、25μg/L BPA和1µg/L E2中24小时进行比较。与对照组相比，暴露于所有剂量BPA和E2的肝组织中erβ-I的mRNA转录物显著增加。在暴露于1和5μg/L BPA和E2的肝组织中，erβ-II的mRNA转录显著增加。用5μg/L BPA和E2处理的肝组织中vtg的mRNA转录物也显著增加。最后，在暴露于5和25μg/L BPA和E2的肝组织中，cyp1a mRNA转录物显著增加。这些结果表明BPA的雌激素活性类似于E2，并表明体外方法可以作为体内测试的替代方法来检测BPA的雌激素作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Fisheries Sciences FISHERIES-

CiteScore

2.30

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： 1- Living various species (contains animals and vegetal species) in various aquatic ecosystems. 2- Health and diseases of aquatic species. 3- Determining the stocks and specific time and location for catching and reliable exploitation for sustainable development. 4- Methods of propagation and culture of high value aquatic resources. 5- Aquatic stock assessment and the methods of restocking the high value species and suggestion for rate, areas and the time for releasing fish and other aquatic organisms fries. 6- Pollutant agents and their effects to the environments of aquatic species. 7- Feed and feeding in aquatic organisms. 8- Fish processing and producing new products. 9- The economic and social aspects of fisheries.