Characteristics of Escherichia coli Isolated from Intestinal Microbiota Children of 0–5 Years Old in the Commune of Abomey-Calavi

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-06-06 DOI:10.1155/2022/6253894

H. Sina, D. Dah-Nouvlessounon, T. Adjobimey, Bawa Boya, Ghislaine M. C. Dohoue, C. N’tcha, Violette Chidikofan, F. Baba-Moussa, I. Abdoulaye, A. Adjanohoun, L. Baba-Moussa

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引用次数: 1

Abstract

Escherichia coli is a commensal bacterium and one of the first bacteria to colonize the digestive tract of newborns after birth. It is characterized by great versatility and metabolic flexibility that allows its survival in different niches. The present study aims at analyzing the diversity of E. coli strains isolated from the intestinal microbiota of children aged from 0 to 5 years in the commune of Abomey-Calavi in Benin. For this purpose, a descriptive and analytical cross-sectional study was conducted. A total of 135 stool samples were collected from the pediatric clinic of Abomey-Calavi. Microbiological analyses were performed according to standard microbiology analytical techniques. The molecular characterization of E. coli was performed by investigating eight genes (dinB, icdA, pabB, polB, putP, trpA, trpB, and uidA) using the PCR technique. The results showed that the average loading rate on stool samples was 3.74 × 107 CFU/g for TAMF. A total of 7 species of bacteria were identified at different proportions: Staphylococcus spp (55.36%), E. coli (14.29%), Klebsiella ornithinolytica (12.5%), Serratia odorifera (5.36%), and Enterobacter aerogenes (5.36%). Interestingly, isolated E. coli presented a resistance of 100% to cefotaxime and aztreonam. In addition, resistances of 95.24% and 50% were observed against erythromycin and nalidixic acid, respectively. The molecular characterization of the isolated E. coli strains allowed us to discover another molecular variation within the isolated strains. Genes encoding the enzymes isocitrate dehydrogenase (icd) and DNA polymerase II (polB) were detected at 96.30% in the isolated E. coli strains. Moreover, the genes encoding the enzymes beta-D-glucuronidase (uidA) and DNA polymerase (dinB) were detected at 88.89% in the isolated E. coli strains. Interestingly, 81.48%, 85.19, 92.59%, and 100% of isolated E. coli strains expressed the genes encoding the enzymes tryptophan synthase subunit A (trpA), proline permease (putP), p-aminobenzoate synthase, and tryptophan synthase subunit B (trpB), respectively. The diversity of E. coli strains reflects the importance of regulatory mechanisms in the adaptation of bacteria to the gut microbiota.

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Abomey-Calavi社区0-5岁儿童肠道菌群分离大肠杆菌特征

大肠杆菌是一种共生细菌，也是新生儿出生后最早在消化道定植的细菌之一。它的特点是具有强大的多功能性和代谢灵活性，可以在不同的生态位中生存。本研究旨在分析从贝宁Abomey-Calavi社区0至5岁儿童肠道微生物群中分离的大肠杆菌菌株的多样性。为此，进行了一项描述性和分析性的横断面研究。从Abomey Calavi的儿科诊所共采集了135份粪便样本。根据标准微生物学分析技术进行微生物学分析。利用PCR技术对8个基因（dinB、icdA、pabB、polB、putP、trpA、trpB和uidA）进行了分子鉴定。结果显示，粪便样本的平均装载率为3.74 × 107 TAMF的CFU/g。共鉴定出7种不同比例的细菌：葡萄球菌属（55.36%）、大肠杆菌属（14.29%）、奥氏克雷伯菌属（12.5%）、气味沙雷氏菌属（5.36%）和产气肠杆菌属（5.36%）。有趣的是，分离的大肠杆菌对头孢噻肟和氨曲南的耐药性为100%。此外，对红霉素和萘啶酸的抗性分别为95.24%和50%。分离的大肠杆菌菌株的分子特征使我们能够在分离的菌株中发现另一种分子变异。在分离的大肠杆菌菌株中，编码异柠檬酸脱氢酶（icd）和DNA聚合酶II（polB）的基因检出率为96.30%。此外，在分离的大肠杆菌菌株中，编码β-D-葡糖醛酸酶（uidA）和DNA聚合酶（dinB）的基因检出率为88.89%。有趣的是，81.48%、85.19%、92.59%和100%的分离大肠杆菌菌株分别表达编码色氨酸合成酶亚基A（trpA）、脯氨酸渗透酶（putP）、对氨基苯甲酸合成酶和色氨酸合酶亚基B（trpB）的基因。大肠杆菌菌株的多样性反映了细菌适应肠道微生物群的调节机制的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.