Functional and structural assessment of the possible protective effect of platelet-rich plasma against ischemia/reperfusion-induced ovarian injury in adult rats

IF 1.4 4区 医学 Q4 PHYSIOLOGY
Eman A Allam, Rehab Ahmed Abdel Moniem, G. Soliman
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引用次数: 1

Abstract

This study aimed to evaluate the possible protective effect of platelet-rich plasma (PRP) on ischemia reperfusion (I/R)-induced ovarian injury in a rat model. Forty adult female albino rats were randomly assigned to four groups: control, ischemia, I/R, and I/R + intraperitoneal PRP. Induction of ischemia was done by bilateral ovarian torsion for 3 h, while reperfusion was done by subsequent detorsion for another 3 h. PRP was injected 30 min before detorsion. Histological assessment and measurement of ovarian anti-Mullerian hormone (AMH) were done to assess the degree of tissue damage and the remaining ovarian reserve. Ovarian malondialdehyde (MDA) and total antioxidant capacity (TAC) levels were measured to evaluate the oxidant-antioxidant balance. Tumor necrosis factor-α (TNF-α) was measured to assess degree of inflammation. Immunohistochemical assessment of ovarian vascular endothelial growth factor-A (VEGF-A) was also done. PRP treated I/R group revealed a significant decrease in MDA (P = 0.007), TNF-α (P = 0.001), and a significant increase in TAC (P = 0.001) and VEGF-A (P = 0.003) in comparison to the untreated I/R group. Furthermore, limited vascular congestion and inflammatory infiltration were observed after PRP treatment. However, no significant difference was detected in AMH after PRP treatment. Our results denoted that PRP may help in preservation of ovarian function and structure during surgical conservative detorsion of the torsioned ovary. These protective effects could be attributed to its ability to reduce oxidative stress, inflammation and also to its high content of growth factors especially VEGF.
富血小板血浆对成年大鼠卵巢缺血再灌注损伤可能保护作用的功能和结构评价
本研究旨在评估富血小板血浆(PRP)对缺血再灌注(I/R)诱导的大鼠卵巢损伤的可能保护作用。将40只成年雌性白化大鼠随机分为四组:对照组、缺血组、I/R组和I/R+腹腔PRP组。通过双侧卵巢扭转诱导缺血3小时,而再灌注则通过随后的排毒再灌注3小时。在排毒前30分钟注射PRP。进行组织学评估和卵巢抗苗勒管激素(AMH)的测量,以评估组织损伤的程度和剩余的卵巢储备。测定卵巢丙二醛(MDA)和总抗氧化能力(TAC)水平,以评估氧化-抗氧化平衡。测定肿瘤坏死因子-α(TNF-α)以评估炎症程度。卵巢血管内皮生长因子-A(VEGF-A)的免疫组化评估也进行了。与未治疗的I/R组相比,PRP治疗I/R组的MDA(P=0.007)、TNF-α(P=0.001)显著降低,TAC(P=0.000)和VEGF-a(P=0.003)显著增加。此外,PRP治疗后观察到有限的血管充血和炎症浸润。然而,PRP治疗后AMH没有发现显著差异。我们的研究结果表明,PRP可能有助于在扭转卵巢的手术保守切除过程中保持卵巢功能和结构。这些保护作用可能归因于其减少氧化应激和炎症的能力,也归因于其高含量的生长因子,尤其是VEGF。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.30
自引率
5.60%
发文量
36
审稿时长
6-12 weeks
期刊介绍: Chinese Journal of Physiology is a multidisciplinary open access journal. Chinese Journal of Physiology (CJP) publishes high quality original research papers in physiology and pathophysiology by authors all over the world. CJP welcomes submitted research papers in all aspects of physiology science in the molecular, cellular, tissue and systemic levels. Multidisciplinary sciences with a focus to understand the role of physiology in health and disease are also encouraged. Chinese Journal of Physiology accepts fourfold article types: Original Article, Review Article (Mini-Review included), Short Communication, and Editorial. There is no cost for readers to access the full-text contents of publications.
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