Thin Layer Chromatography Fingerprint Analysis of Tempuyung (Sonchus arvensis L.)

Sri Fauziah, Puput Melati, A. H. Mulyati, M. Rafi, E. Rohaeti
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引用次数: 0

Abstract

Thin layer chromatography (TLC) fingerprint profile analysis can be used for quality control of herbal medicinal raw materials through identification, authentication, and discrimination. This study aims to develop a fingerprint analysis method for tempuyung TLC (Sonchus arvensis L.), which is then used for quality control. Tempuyung was extracted with methanol using ultrasonication which was then analyzed using the developed fingerprint TLC method. The optimum mobile phase used to separate compounds from tempuyung was a composition of chloroform: ethyl acetate: dichloromethane: formic acid (7.5:2:0.5:0.1) and produced eleven bands. The mobile phase composition produced good separation and had a typical luteolin band with an Rf value of 0.22, detected under UV 366 nm and derivatized with 10% sulfuric acid reagent. This method was applied to tempuyung from three locations, including Malang, Solo, and Yogyakarta, whose fingerprint patterns did not differ significantly. The fingerprint method has been validated and met the acceptance requirements for quality control of tempuyung.
天普雍药材的薄层指纹图谱分析
薄层色谱(TLC)指纹图谱分析可用于中药材原料的鉴别、鉴别、鉴别等质量控制。本研究旨在建立天普雍薄层色谱的指纹图谱分析方法,并用于质量控制。采用甲醇超声提取坦普雍,并采用建立的指纹薄层色谱法对其进行分析。以氯仿:乙酸乙酯:二氯甲烷:甲酸(7.5:2:0.5:0.1)为最佳流动相,得到了11条色谱带。流动相组成分离效果好,具有典型的木犀草素带,Rf值为0.22,在366 nm紫外下检测,用10%硫酸试剂衍生。将该方法应用于玛琅、梭罗和日惹3个地点的坦普雍,其指纹图谱差异不显著。指纹图谱法经验证,符合坦普永质量控制验收要求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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17 weeks
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