Expression analysis of POSTN gene in ovine follicles

IF 1.2 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Canadian Journal of Animal Science Pub Date : 2022-10-26 DOI:10.1139/cjas-2021-0036

Jiapeng Lin, Chunjie Liu, Liqin Wang, Ying Chen, Xiaolin Li, Yangsheng Wu, Juncheng Huang

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Abstract

Abstract The purpose of this study was to explore the potential expression regularity of POSTN in ovarian tissue. In this study, the uterus, ovarian follicles, heart, liver, spleen, lung, kidney, and muscle tissues of Merino sheep (10) were collected and detected by Western blot, real-time quantitative polymerase chain reaction, immunohistofluorescence, and fluorescence staining, respectively. The expression of POSTN in various organizations was analyzed. The results showed that the POSTN in Merino sheep had close homology with Bos mutus and Bos taurus. The expression of POSTN was detected in the uterus, follicle, heart, liver, spleen, lung, kidney, and muscle tissue, among which the expression of POSTN was highest in ovarian tissue. In addition, the expression of POSTN gradually increased with the increase of follicle diameter, among which POSTN was highly expressed in the granulosa cells (GCs) of follicles. Meanwhile, POSTN were distributed throughout the nucleus and cytoplasm of GCs, suggesting that POSTN may be involved in the regulation of follicle development.

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POSTN基因在绵羊卵泡中的表达分析

摘要本研究旨在探讨POSTN在卵巢组织中的潜在表达规律。在本研究中，收集美利奴羊（10）的子宫、卵泡、心、肝、脾、肺、肾和肌肉组织，并分别通过蛋白质印迹、实时定量聚合酶链式反应、免疫组织荧光和荧光染色进行检测。分析了POSTN在不同组织中的表达。结果表明，美利奴羊的POSTN基因与麋鹿和牛头犬具有密切的同源性。POSTN在子宫、卵泡、心、肝、脾、肺、肾和肌肉组织中表达，其中POSTN在卵巢组织中表达最高。此外，POSTN的表达随着卵泡直径的增加而逐渐增加，其中POSTN在卵泡颗粒细胞（GC）中高表达。同时，POSTN分布于GC的细胞核和细胞质中，提示POSTN可能参与卵泡发育的调控。

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来源期刊

Canadian Journal of Animal Science 农林科学-奶制品与动物科学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Published since 1957, this quarterly journal contains new research on all aspects of animal agriculture and animal products, including breeding and genetics; cellular and molecular biology; growth and development; meat science; modelling animal systems; physiology and endocrinology; ruminant nutrition; non-ruminant nutrition; and welfare, behaviour, and management. It also publishes reviews, letters to the editor, abstracts of technical papers presented at the annual meeting of the Canadian Society of Animal Science, and occasionally conference proceedings.