In-vitro Antioxidant and In-vitro Anti-inflammatory activities of Ethanolic leaves extract of Ormocarpum Cochinchinense

Abstract

Introduction: Periodontitis, a chronic inflammatory disease with microbial etiology, is mediated by multiple inflammatory processes and oxidative stress is now well recognized as a part of periodontal pathogenesis. A balance between reactive oxygen species and antioxidants is required to maintain periodontal health. Medicinal herbs with bioactive phytocompounds have rich source of antioxidants and anti-inflammatory compounds. Ormocarpum cochinchinense is a medicinal herb with antioxidants and anti-inflammatory phytocompounds. The phytocompounds activities of the herb are not much explored. This study is focused on the In-vitro antioxidant and anti-inflammatory activities of the ethanolic extract of leaves of O. cochinchinense. To assess the In-vitro antioxidant and In-vitro anti-inflammatory activities of ethanolic extracts of O. cochinchinense. Materials and Methods: The leaves of O. cochinchinense were collected, air dried in the shade, and then powdered in an electric blender. The preparation of ethanolic extract was carried out. In-vitro antioxidant studies using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Nitric Oxide (NO) assays along with anti-inflammatory activity by protein denaturation inhibition and membrane stabilization method were studied. Descriptive statistics were used for continuous variables and expressed in mean and standard deviation. One way ANOVA with post-hoc tukey test or Kruskal–Wallis test, Post-hoc Mann–Whitney U test was used according to the normal distribution of the sample. To compare the individual study group against their standard group, independent t test, and Mann–Whitney U test have been used. P < 0.05 was considered significant. Results: O. cochinchinense had significant antioxidant and anti-inflammatory activities. The ethanolic extract showed dose-dependant activity in all analyses performed (P < 0.05). NO inhibition assay showed 95% of antioxidant activity and 80% of anti-inflammatory activity in the Human Red Blood Cell (HRBC) Membrane Stabilization assay. Conclusions: O. cochinchinense could be used as an adjuvant supplement to conventional therapy in the treatment of chronic inflammatory diseases.