Evaluation of In-vitro Cytoprotective, Wound Healing and Antioxidant Effects of Ocimum sanctum Leaf ExtractChetna

Q3 Pharmacology, Toxicology and Pharmaceutics

Current Trends in Biotechnology and Pharmacy Pub Date : 2021-08-18 DOI:10.5530/CTBP.2021.3.27

Chetna Faujdar, Priyadarshini

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引用次数: 1

Abstract

Exposure of renal epithelial cells to Calcium oxalate monohydrate crystals leads to cellular injury and play a significant role in the formation of kidney stones. Additionally, lipid peroxidation of polyunsaturated fatty acids further accelerates the generation and progression of the disease. Availability of safe and effective treatment of the disease is the need of hour. The objective of this research work was to evaluate the impact of hydro-alcoholic extract of Ocimum sanctum leaves on renal epithelial cell injury, oxidative stress and wound healing. Renal epithelial cells (Vero cells) were exposed to Calcium oxalate monohydrate crystals to cause injury and then injured cells were treated with Ocimum sanctum extract. Cell viability of the treated group was compared against control using MTT assay. Antioxidant potential was evaluated using malondialdehyde content as biomarker. Wound healing potential was evaluated using scratch assay. A significant increase in cell viability and wound closure rate was observed in the treated group compared to that in control group was observed. We observed reduced malondialdehyde content in the treated group as compared with control. It can be concluded that hydro-alcoholic extract of leaves of Ocimum sanctum possesses strong cytoprotective, antioxidant and wound healing potential and can be effective in the prevention and treatment of kidney stones.

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桑叶提取物Chetna的体外细胞保护、创伤愈合和抗氧化作用评价

肾上皮细胞暴露于一水草酸钙晶体可导致细胞损伤，并在肾结石的形成中起重要作用。此外，多不饱和脂肪酸的脂质过氧化进一步加速了疾病的发生和发展。目前需要一个小时的时间来获得安全有效的治疗方法。本研究的目的是评价山茱萸叶水酒精提取物对肾上皮细胞损伤、氧化应激和伤口愈合的影响。将肾上皮细胞(Vero细胞)暴露于一水草酸钙晶体中造成损伤，然后用神荆提取物处理损伤细胞。用MTT法比较处理组和对照组的细胞活力。以丙二醛含量为生物标志物评价抗氧化潜力。采用划痕法评价创面愈合潜力。与对照组相比，治疗组细胞活力和创面愈合率显著提高。我们观察到治疗组与对照组相比丙二醛含量降低。由此可见，茴香叶水酒精提取物具有较强的细胞保护、抗氧化和伤口愈合潜力，可有效预防和治疗肾结石。

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来源期刊

Current Trends in Biotechnology and Pharmacy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： The Association of Biotechnology and Pharmacy (ABAP) will be useful to form a forum for scientists so that they can bring together to discuss and find scientific solutions to the problems of society. The annual meetings will help the members to share their knowledge and publish their research knowledge particularly by members and fellows of the Association and special care will be taken to provide an opportunity for young scientists. Besides this the association is planned to organize symposia, seminars and workshops on current developments of Biotechnology and Pharmacy particularly on the subject of current scientific interest, and the proceedings of which will be published regularly. And in view of the vast development of science and to disseminate the problems in publication of research work, an international journal of Current Trends in Biotechnology and Pharmacy has been started by ABAP.