Christine D. Kuslich, Buena Chui, Carl T. Yamashiro
{"title":"Overview of PCR","authors":"Christine D. Kuslich, Buena Chui, Carl T. Yamashiro","doi":"10.1002/cpet.27","DOIUrl":null,"url":null,"abstract":"<p>The polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique previously. In addition, a number of different applications utilizing the basic PCR technique will be described. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":"18 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpet.27","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpet.27","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
The polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique previously. In addition, a number of different applications utilizing the basic PCR technique will be described. © 2018 by John Wiley & Sons, Inc.
PCR概述
聚合酶链反应(PCR)彻底改变了分子生物学，并使许多其他技术和应用成为可能，这些技术和应用通常用于研究中，并且是许多生命科学产品的一部分。本单元将涵盖PCR的基本原理，包括如何在以前没有使用这种强大技术的实验室中进行PCR。此外，利用基本PCR技术的一些不同的应用将被描述。©2018 by John Wiley & Sons, Inc。
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