Evaluation of treatment methods for spiking deoxynivalenol (DON) in single corn kernels

IF 1 4区农林科学 Q3 AGRONOMY

Canadian Journal of Plant Science Pub Date : 2023-05-05 DOI:10.1139/cjps-2022-0259

T. S. Rathna Priya, A. Manickavasagan

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引用次数: 0

Abstract

Current research on deoxynivalenol (DON), a trichothecene secondary metabolite produced by the Fusarium species in corn grains, relies on the time-consuming field inoculation of Fusarium species with unpredictable and low recovery rates of DON in grains. The objective of this study was to evaluate three methods: soaking whole grains, soaking cracked grains, and injection in three solvents (distilled water, methanol, and acetonitrile) at three toxin concentration levels (1, 5, and 10 µg/g) for facilitating DON absorption in corn grains. The effectiveness of each treatment method and the performance of each solvent in aiding DON absorption were analysed and compared with the recovery rates of DON in the treated corn grains. The treatment methods, solvent, and DON concentration in solvent had significant effect on the recovery rate of DON in treated kernels. Injecting whole grains showed the highest recovery rates of DON (60%–108%) followed by soaking cracked grains (10%–87%) and whole grain (10%–72%) treatment methods. Distilled water showed the highest recovery rates in both soaking (53%–87%) and injection (74%–105%) treatment methods followed by methanol (18%–68% for soaking; 66%–103% for injection) and acetonitrile (10%–36% for soaking; 61%–108% for injection). Water dispersed the arrangement of starch granules but caused no changes in their surface morphology. Methanol and acetonitrile showed disruptive effects on the surface morphology of starch granules.

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玉米单粒萌发脱氧雪腐镰刀菌醇(DON)处理方法的评价

脱氧雪腐镰刀菌醇(deoxynivalenol, DON)是镰刀菌在玉米籽粒中产生的一种毛霉烯次生代谢物，目前的研究依赖于田间接种时间长、籽粒DON回收率低且不可预测的镰刀菌。本研究的目的是评估三种方法:浸泡全谷物、浸泡破碎谷物和注射三种溶剂(蒸馏水、甲醇和乙腈)在三种毒素浓度水平(1、5和10µg/g)下促进玉米籽粒对DON的吸收。分析了各处理方法的效果和各溶剂对DON的吸附性能，并与处理后玉米籽粒中DON的回收率进行了比较。处理方式、溶剂、溶剂中DON浓度对处理后籽粒中DON的回收率有显著影响。注射全粒处理的DON回收率最高(60% ~ 108%)，其次是浸泡裂粒处理(10% ~ 87%)和全粒处理(10% ~ 72%)。蒸馏水浸泡法和注射法回收率最高(53% ~ 87%)，其次是甲醇浸泡法(18% ~ 68%);注射66%-103%)、乙腈(浸泡10%-36%);注射61% ~ 108%)。水分散了淀粉颗粒的排列，但没有引起淀粉颗粒表面形态的变化。甲醇和乙腈对淀粉颗粒表面形貌有破坏作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Canadian Journal of Plant Science 生物-农艺学

CiteScore

1.90

自引率

8.30%

发文量

审稿时长

1 months

期刊介绍： Published since 1957, the Canadian Journal of Plant Science is a bimonthly journal that contains new research on all aspects of plant science relevant to continental climate agriculture, including plant production and management (grain, forage, industrial, and alternative crops), horticulture (fruit, vegetable, ornamental, greenhouse, and alternative crops), and pest management (entomology, plant pathology, and weed science). Cross-disciplinary research in the application of technology, plant breeding, genetics, physiology, biotechnology, microbiology, soil management, economics, meteorology, post-harvest biology, and plant production systems is also published. Research that makes a significant contribution to the advancement of knowledge of crop, horticulture, and weed sciences (e.g., drought or stress resistance), but not directly applicable to the environmental regions of Canadian agriculture, may also be considered. The Journal also publishes reviews, letters to the editor, the abstracts of technical papers presented at the meetings of the sponsoring societies, and occasionally conference proceedings.