Chemical composition, antioxidant and antibacterial activities from methanolic extract of Crataegus monogyna Jacq. (Rosaceae)

Abstract

The monogynous hawthorn or Crataegus monogyna Jacq (Rosaceae) is a medicinal plant used since antiquity in traditional medicine, recognized by its therapeutic virtues. This study aims to estimate the total phenols and flavonoids contents and to evaluate the antioxidant and antibacterial activity in vitro from the methanolic extract of the C. monogyna leaves. Antioxidant activity was investigated with six methods, DPPH, ABTS, galvinoxyl scavenging activities. FRAP, CUPRAC and phenanthroline assays , Total phenols and flavonoids contents were studied by Folin–Ciocalteau and aluminum colorimetric methods. The antibacterial activity of the methanolic extract was evaluated against Staphylococcus aureus , Proteus mirabilis , Pseudomonas aeruginosa , Escherichia coli and Streptococcus Sp in the base of inhibition zone diameter using agar diffusion method . The findings showed a significant antioxidant activity by the methods of DPPH (IC 50 =28.00±0.97 μ g/ml), CUPRAC (A 0.5 =28.79± 2.10µg/ml), ABTS (IC 50 =10.12±0.60µg/ml), phenanthroline (A 0.5 =36.84±1.85µg/ml), GOR (IC 50 = 20.76±0.40 μ g/ml) but this activity is absent with FRAP assay. The amount of TPC was very high in this extract (201.47±0.55 µg EAG/mg), while the value of flavonoids was moderate (75.48 ±5.77µg EQ/mg). The methanolic extract of C. monogyna leaves showed a significant antibacterial potency. The present study has demonstrated that C. monogyna extract possess potent antioxidant capacity and a good antibacterial activity, which could be include in medicinal use.