Dendrobium nobile Lindl polysaccharides improve testicular spermatogenic function in streptozotocin-induced diabetic rats

IF 2.7 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Reproduction and Development Pub Date : 2022-03-20 DOI:10.1002/mrd.23556

Xiaocan Lei, Peng Huo, Yuan-jie Xie, Yaohui Wang, Guanghai Liu, Haoyan Tu, Qingxiang Shi, Zhong-cheng Mo, Shun Zhang

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引用次数: 6

Abstract

Dendrobium nobile Lindl polysaccharides (DNLP) exhibited various biological functions. This study aimed to investigate the protective effects of DNLP on testicular spermatogenic function in streptozotocin (STZ)-induced diabetic rats in comparison with metformin. The blood glucose level was significantly increased and the homeostatic model assessment for insulin resistance (HOMA-IR) aggravated markedly in diabetic rats. The weight of testis and epididymis, and the sperm number and motility were decreased in the diabetic rats. The pathologic changes occurred in the spermatogenic tubules along with the decreased number of spermatogenic cells, downregulated proliferating cell nuclear antigen (PCNA) and Sirtuin 1 (SIRT1) expression and increased cell apoptosis in the testes. Compared with the model group, DNLP and metformin treatment significantly decreased the level of blood glucose, improved the HOMA-IR, and increased the weight of testis and epididymis, as well as the sperm number and sperm motility. Furthermore, the pathologic changes in the spermatogenic tubules improved significantly with increased number of spermatogenic cells, the upregulation of PCNA and SIRT1 and suppression of cell apoptosis in the testes. Collectively, our study for the first time examined the effects of DNLP on the male reproductive system of STZ-induced diabetic rats, and indicated that DNLP was protective against diabetes mellitus-induced testis injury via increasing the proliferation, inhibiting cell apoptosis and upregulating SIRT1 expression in testicular spermatogenic cells.

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石斛多糖改善链脲佐菌素诱导的糖尿病大鼠睾丸生精功能

石斛多糖(DNLP)具有多种生物学功能。本研究旨在探讨DNLP对链脲佐菌素(STZ)诱导的糖尿病大鼠睾丸生精功能的保护作用，并与二甲双胍进行比较。糖尿病大鼠血糖水平显著升高，胰岛素抵抗稳态模型评估(HOMA‐IR)显著加重。糖尿病大鼠睾丸、附睾重量下降，精子数量和活力下降。睾丸生精小管发生病变，生精细胞数量减少，增殖细胞核抗原(PCNA)和Sirtuin 1 (SIRT1)表达下调，细胞凋亡增加。与模型组比较，DNLP和二甲双胍治疗显著降低大鼠血糖水平，改善HOMA‐IR，增加睾丸和附睾重量、精子数量和精子活力。此外，随着睾丸生精细胞数量的增加、PCNA和SIRT1的上调以及细胞凋亡的抑制，生精小管的病理改变明显改善。总之，我们的研究首次检测了DNLP对STZ诱导的糖尿病大鼠雄性生殖系统的影响，表明DNLP通过增加睾丸生精细胞的增殖、抑制细胞凋亡和上调SIRT1表达，对糖尿病诱导的睾丸损伤具有保护作用。

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来源期刊

Molecular Reproduction and Development 生物-发育生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Molecular Reproduction and Development takes an integrated, systems-biology approach to understand the dynamic continuum of cellular, reproductive, and developmental processes. This journal fosters dialogue among diverse disciplines through primary research communications and educational forums, with the philosophy that fundamental findings within the life sciences result from a convergence of disciplines. Increasingly, readers of the Journal need to be informed of diverse, yet integrated, topics impinging on their areas of interest. This requires an expansion in thinking towards non-traditional, interdisciplinary experimental design and data analysis.