Detection of Quorum Sensing System (Cell to Cell Communication) Using Marker Strains in Vibrio alginolyticus Strains and Determine Virulence under Master of this System

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-01-25 DOI:10.12681/jhvms.28525

N. Filik, A. Kubilay

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引用次数: 0

Abstract

ABSTRACTThere are intercellular communication and social behaviors, including Quorum Sensing System (QS) in bacteria. The present study aims to investigate the production of QS signal molecules (N-acyl homoserine lactones, AHLs) in bacterial fish pathogen Vibrio alginolyticus (3 strains) and also determine the virulence using these AHLs under the management of the QS in V. alginolyticus. QS of V. alginolyticus was proved by cross validation assay used by AHL marker bacteria strains (Agrobacterium tumefaciens NT1 and Chromobacterium violaceum CV026). V. alginolyticus was not produced N-butanoyl homoserine lactone (BHL), while N-(3-okzododekanoyl)-L-homoserine lactone (OdDHL) production was detected in V. alginolyticus. The presence of virulence factors, such as biofilm, pigment, rhamnolipid, hemolysis, elastase, protease and amylase production, was investigated as phenotypically in V. alginolyticus. V. alginolyticus was found to produce biofilm, pigment, amylase and elastase; however, it was not determined to produce rhamnolipid, hemolysis and protease virulence.

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利用标记菌株检测溶藻弧菌群体感应系统(细胞间通讯)并在该系统下测定毒力

摘要细菌中存在细胞间通讯和社会行为，包括群体感应系统。本研究旨在研究细菌性鱼类病原体溶藻弧菌（3株）中QS信号分子（N-酰基高丝氨酸内酯，AHL）的产生，并确定在溶藻弧菌QS管理下使用这些AHL的毒力。AHL标记菌菌株（根癌农杆菌NT1和紫色杆菌CV026）的交叉验证试验证明了溶藻弧菌的QS。溶藻弧菌不产生N-丁酰基高丝氨酸内酯（BHL），而在溶藻弧菌中检测到N-（3-氧唑酮酰基）-L-高丝氨酸内酯的产生。毒力因子的存在，如生物膜、色素、鼠李糖脂、溶血、弹性蛋白酶、蛋白酶和淀粉酶的产生，被研究为溶藻弧菌的表型。溶藻弧菌能产生生物膜、色素、淀粉酶和弹性蛋白酶；但未测定其产生鼠李糖脂、溶血和蛋白酶毒力。

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来源期刊

Journal of the Hellenic Veterinary Medical Society VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of the Hellenic Veterinary Medical Society (J Hellenic Vet Med Soc) is a quarterly peer-reviewed journal that publishes articles in all aspects of veterinary science and related disciplines. It is published by the Hellenic Veterinary Medical Society and is indexed in the Web of Science and in Scopus. There are no publication fees in the journal. Authors considering submitting manuscripts for evaluation and publication are requested to read carefully the instructions for authors and fully comply with them. Non-complying manuscripts may be returned to the corresponding author for formatting.