Dendritic polyglycerols are modulators of microglia-astrocyte crosstalk

IF 0.6 Q4 CLINICAL NEUROLOGY

Future Neurology Pub Date : 2019-11-01 DOI:10.2217/fnl-2019-0008

D. Maysinger, Mélanie Lalancette–Hébert, Jeff Ji, K. Jabbour, J. Dernedde, K. Silberreis, R. Haag, J. Kriz

引用次数: 10

Abstract

Aim: To determine the ability of sulfated dendritic polyglycerols (dPGS) to modulate neuroglia activation challenged with lipopolysaccharide (LPS). Materials & methods: Microglia/astrocyte activation in vivo was determined in transgenic animals expressing TLR2-/GFAP-luciferase reporter. Mechanisms implicated in microglia-astrocyte crosstalk were studied in primary mouse brain cultures. Results & discussion: dPGS significantly reduced microglia activation in vivo, and decreased astrocytic LCN2 production. Activated microglia are necessary for astrocyte stimulation and increase in LCN2 abundance. LCN2 production in astrocytes involves signaling via toll-like receptor 4, activation of NF-κB, IL6 and enhancement of reactive oxygen species. Conclusion: dPGS are powerful modulators of microglia-astrocyte crosstalk and LCN2 abundance; dPGS are promising anti-inflammatory dendritic nanostructures.

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树突状聚甘油是小胶质细胞-星形胶质细胞串扰的调节剂

目的:探讨硫酸树突状聚甘油(dPGS)对脂多糖(LPS)诱导的神经胶质细胞活化的调节作用。材料与方法:在表达TLR2-/ gmap -荧光素酶报告基因的转基因动物体内检测小胶质细胞/星形胶质细胞的激活情况。在小鼠原代脑培养中研究了小胶质细胞-星形胶质细胞串扰的机制。结果与讨论:dPGS在体内显著降低小胶质细胞的激活，并减少星形胶质细胞LCN2的产生。激活的小胶质细胞是星形胶质细胞刺激和LCN2丰度增加所必需的。星形胶质细胞中LCN2的产生涉及通过toll样受体4、NF-κB、il - 6的激活和活性氧的增强来传递信号。结论:dPGS是小胶质-星形胶质细胞串扰和LCN2丰度的有效调节剂;dPGS是一种很有前途的抗炎树突纳米结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future Neurology CLINICAL NEUROLOGY-

CiteScore

2.10

自引率

0.00%

发文量

期刊介绍： The neurological landscape is changing rapidly. From the technological perspective, advanced molecular approaches and imaging modalities have greatly increased our understanding of neurological disease, with enhanced prospects for effective treatments in common but very serious disorders such as stroke, epilepsy, multiple sclerosis and Parkinson’s disease. Nevertheless, at the same time, the healthcare community is increasingly challenged by the rise in neurodegenerative diseases consequent upon demographic changes in developed countries.