DYNAMICS OF LEAD TOLERANCE IN TOBACCO (NICOTIANA TABACUM L.) GENOTYPES

IF 1.6 Q3 PLANT SCIENCES

Sabrao Journal of Breeding and Genetics Pub Date : 2023-08-31 DOI:10.54910/sabrao2023.55.4.25

M. M. Hussain, A. Saeed, M. Shakeel, A. Rauf, S. Gul, M. Mohibullah, M. Munir, I. Khan, M. Yasin, Dr. Sajjad Hussain Qureshi

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引用次数: 0

Abstract

Lead nitrate has reports of significantly inhibiting plant growth. Early exploration of the genotypic difference for lead nitrate stress in tobacco has started. The presented study had eight tobacco genotypes subjected to 200 μM lead nitrate (Pb [NO3]2) stress in a hydroponic culture. Lead stress treatment to plants for 14 days had data recording at three times intervals of stressed plants. Assessing photosynthetic and antioxidant enzymes’ activities was in a time series order of one day, seven days, and 14 days. One-day, seven-day, and fourteen-day-old seedlings gained treatment of 200 μM lead nitrate stress and control. Soil Plant Analysis Development (SPAD) values for most genotypes decreased, while oxidant and anti-oxidant enzymes increased activity. Chlorophyll-a, chlorophyll-b, and total chlorophyll evaluated after lead nitrate toxicity showed reduced activity in studied tobacco genotypes compared with control as time passed. All chlorophyll contents, i.e., chlorophyll a, b, and total chlorophyll, declined with a longer span in lead nitrate solution. Genotype QVA-20 could benefit lead-salt tolerance and susceptible genotype ‘long chang’ cigarette based on chlorophyll content and SPAD values. Chlorophyll a capacity decreased as lead exposure to plants increased, but chlorophyll b increased in all genotypes on the 15th day. The MDA (malondialdehyde) content increased in all tobacco genotypes with increased lead nitrate exposure. Meanwhile, SOD (superoxide dismutase) contents decreased in genotypes RG-8, E1, and X6 with increased time, but POD (peroxidase) contents increased in all genotypes on the 14th day. Genotypes RG-8, E1, and X6 proved considerably tolerant of lead toxicity at 200 µM.

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烟草（nicotianatabacuml.）基因型对铅的耐受动态

硝酸铅有显著抑制植物生长的报道。烟草硝酸铅胁迫基因型差异的早期探索已经开始。本研究有八种烟草基因型在水培培养中受到200μM硝酸铅（Pb[NO3]2）胁迫。对植物进行铅胁迫处理14天，以胁迫植物的三倍间隔记录数据。评估光合酶和抗氧化酶的活性是按照一天、七天和14天的时间序列顺序进行的。一日龄、七日龄和十四日龄幼苗分别接受200μM硝酸铅胁迫处理和对照。大多数基因型的土壤植物分析发育（SPAD）值降低，而氧化酶和抗氧化酶的活性增加。在硝酸铅毒性后评估的叶绿素a、叶绿素b和总叶绿素显示，与对照相比，随着时间的推移，所研究的烟草基因型的活性降低。在硝酸铅溶液中，所有叶绿素含量，即叶绿素a、b和总叶绿素，都随着时间的延长而下降。根据叶绿素含量和SPAD值，基因型QVA-20有利于铅耐盐性和易感基因型“长昌”香烟。叶绿素a容量随着铅暴露量的增加而降低，但在第15天，所有基因型的叶绿素b都增加了。所有烟草基因型的MDA（丙二醛）含量随着硝酸铅暴露量的增加而增加。同时，基因型RG-8、E1和X6的SOD（超氧化物歧化酶）含量随着时间的增加而降低，但在第14天，所有基因型的POD（过氧化物酶）含量都增加。RG-8、E1和X6基因型被证明在200µM下对铅毒性具有相当大的耐受性。

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来源期刊

Sabrao Journal of Breeding and Genetics 农林科学-奶制品与动物科学

CiteScore

1.90

自引率

50.00%

发文量

期刊介绍： The SABRAO Journal of Breeding and Genetics is an international journal of plant breeding and genetics research and was first published in 1969. It is the official publication of the Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO). Its objectives are to: promote the international exchange of research information on plant breeding and genetics, by describing new research findings, or ideas of a basic or practical nature; and be a medium for the exchange of ideas and news regarding members of the Society. The Journal gives priority to articles that are of direct relevance to plant breeders and with emphasis on the Asian region. Invited for publication are research articles, short communications, methods, reviews, commentaries, and opinion articles. Scientific contributions are refereed and edited to international standards. The journal publishes articles for SABRAO members mainly. The Journal preferred strongly that at least one author should be a current member of the Society. Non-members may also publish in the journal.