Insights into the systematics of Alluaudinella and allied Aethiopomyia and Ochromusca (Muscidae, Diptera)

IF 2.3 2区生物学 Q2 EVOLUTIONARY BIOLOGY

Zoologica Scripta Pub Date : 2023-02-21 DOI:10.1111/zsc.12584

K. Walczak, T. Pape, M. Ekanem, K. Szpila, A. Grzywacz

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引用次数: 0

Abstract

The muscid genera Alluaudinella Giglio‐Tos, 1895, Aethiopomyia Malloch, 1921 and Ochromusca Malloch, 1927 form a monophyletic group supported by immature and adult morphology and a highly specialised snail‐feeding strategy of immature stages. In contrast to the undoubted monophyly of the Alluaudinella‐Aethiopomyia‐Ochromusca clade, previous studies have provided contradictory hypotheses of the subfamiliar position within the Muscidae, and these three genera have been placed in the subfamily Muscinae, Dichaetomyiinae, Phaoniinae and Reinwardtiinae. The systematic position of Alluaudinella, as a representative of Alluaudinella, Aethiopomyia and Ochromusca group, is revised by means of larval morphology, biology and molecular data. Light microscopy (LM), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) are used to study the egg and all larval instars of Alluaudinella flavicornis (Macquart, 1855) and a multilocus Sanger sequencing (mS‐seq) approach to examine position within Muscidae. Results are inconsistent with the traditional, morphology‐based concept of the Alluaudinella‐Aethiopomyia‐Ochromusca clade as closely related to Dichaetomyia Malloch, 1921, and the phylogenetic analysis revealed no support for inclusion within subfamily Phaoniinae. Larval morphology in Alluaudinella differs significantly from that of Dichaetomyia (and other Phaoniinae), but resembles that of genera nested in Reinwardtiinae. Based on molecular data and larval morphology a transfer of Alluaudinella, Aethiopomyia and Ochromusca to the subfamily Reinwardtiinae is proposed.

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黑蝇亚目及其亲缘类黑蝇亚目的系统学研究(蝇科，双翅目)

肌肉属Alluaudinella Giglio‐Tos，1895年，Aethiopomyia Malloch，1921年和Ochromusca Malloc，1927年形成了一个由未成熟和成年形态以及未成熟阶段高度专业化的蜗牛喂养策略支持的单系群。与Alluaudinella‐Aethiopomyia‐Ochromusca分支的毋庸置疑的单系相反，先前的研究对蝇科中的亚科位置提供了相互矛盾的假设，这三个属被归入蝇亚科、双蝇亚科、Phaoniinae亚科和Reinwardtiinae亚科。利用幼虫形态、生物学和分子数据，对作为Alluaudinella、Aethiopomyia和Ochromusca类群代表的Alluaudidella的系统地位进行了修正。利用光学显微镜（LM）、共聚焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）研究了黄腹蠊（Macquart，1855）的卵和所有幼虫龄，并采用多点Sanger测序（mS-seq）方法检测了其在蝇科中的位置。结果与Alluaudinella‐Aethiopomyia‐Ochromusca分支的传统形态学概念不一致，Alluaudidella‐Ae硫代pomyia‐OChromuska分支与Dichaetomyia Malloch（1921）密切相关，系统发育分析显示不支持包含在Phaoniinae亚科中。Alluaudinella的幼虫形态与Dichaeomyia（和其他Phaoniinae）有显著差异，但与Reinwardtiinae中嵌套的属相似。根据分子数据和幼虫形态，提出了将Alluaudinella、Aethiopomyia和Ochromusca转移到Reinwardtiinae亚科。

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来源期刊

Zoologica Scripta 生物-动物学

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Zoologica Scripta publishes papers in animal systematics and phylogeny, i.e. studies of evolutionary relationships among taxa, and the origin and evolution of biological diversity. Papers can also deal with ecological interactions and geographic distributions (phylogeography) if the results are placed in a wider phylogenetic/systematic/evolutionary context. Zoologica Scripta encourages papers on the development of methods for all aspects of phylogenetic inference and biological nomenclature/classification. Articles published in Zoologica Scripta must be original and present either theoretical or empirical studies of interest to a broad audience in systematics and phylogeny. Purely taxonomic papers, like species descriptions without being placed in a wider systematic/phylogenetic context, will not be considered.