Comparative evaluation of solvent extracts of Azanza garckeana fruit pulp on hormonal profiles, spermiogram and antioxidant activities in rabbit bucks

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2022-12-29 DOI:10.4995/wrs.2022.17256

J. I. Itodo, J. Ayo, I. Rekwot, T. Aluwong, L. Allam, S. Ibrahim

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引用次数: 1

Abstract

The study investigated the comparative influence of different extraction solvents on spermiogram, hormonal profiles and antioxidant activities in rabbit bucks. Adult New Zealand White rabbit bucks (n=18), with average live weight of 1.2±0.03 kg and aged 10-18 mo were fed ad libitum on a commercial diet. They were administered five different Azanza garckeana (AG) fruit pulp extracts at 500 mg/kg via oral gavage, comprising control group (Con), crude (AG Cr), methanol (AG M), n-hexane (AG H), ethyl acetate (AG E) and aqueous (AG AQ) for four weeks. The extracts improved the spermiogram in rabbit bucks administered methanol (AG M) and the reaction time was significantly (P<0.05) lower in AG E group when compared to other groups. The ejaculate volume, sperm motility, pH and sperm concentration were significantly (P<0.05) higher in the AG M group when compared to the other groups. There was a significant (P<0.05) increase in concentrations of blood testosterone, follicle-stimulating hormone and luteinising hormone in methanol extract group (AG M). While the glutathione and malondialdehyde concentrations were (P<0.05) lower, catalase and superoxide dismutase activities were significantly (P<0.05) higher in the groups administered methanol extract (AG M). It was concluded that AG M extracts of AG pulp elicited the best response in spermiogram, hormonal concentrations and antioxidant activities in New Zealand White rabbit bucks. Its use as the extraction solvent is recommended.

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阿扎果肉溶剂提取物对家兔激素、精子图和抗氧化活性的比较评价

研究了不同提取溶剂对兔精子图、激素谱和抗氧化活性的影响。成年新西兰大白兔（n=18），平均活重1.2±0.03 kg，年龄10-18个月，在商业日粮中随意喂养。他们以500mg/kg的剂量通过灌胃给药五种不同的阿扎（AG）果肉提取物，包括对照组（Con）、粗品（AG-Cr）、甲醇（AGM）、正己烷（AGH）、乙酸乙酯（AGE）和水溶液（AG-AQ），持续四周。提取物改善了给予甲醇（AGM）的家兔精子图，与其他组相比，AGE组的反应时间显著缩短（P<0.05）。AG M组的射精量、精子活力、pH值和精子浓度均显著高于其他组（P<0.05）。甲醇提取物组（AGM）血睾酮、卵泡刺激素和黄体生成素浓度显著升高（P＜0.05）。甲醇提取物组的谷胱甘肽和丙二醛浓度较低（P<0.05），过氧化氢酶和超氧化物歧化酶活性显著较高（P<0.05）。结果表明，AG浆中的AG M提取物在精子图、激素浓度和抗氧化活性方面对新西兰大白兔的反应最好。建议将其用作萃取溶剂。

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.