Binding to Intestinal Epithelial Cells and Anti-Inflammatory Activity of Lactobacillus fermentum PL9988

Q4 Medicine

International Journal of Probiotics and Prebiotics Pub Date : 2022-01-11 DOI:10.37290/ijpp2641-7197.17:14-20

J. Paek, B. Kang, H. Yang, Y. Lee

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引用次数: 0

Abstract

Lactobacillus fermentum PL9988 was isolated from an elderly person over 80 years of age living in a Korean longevity village. Results from a previous study showed various characteristics of L. fermentum PL9988 as a probiotic, including resistance to acid and bile acid, immune-enhancing activity, adhesiveness to the intestinal cell line Caco-2, inhibition of various intestinal pathogens, antioxidation activity, and susceptibility to antimicrobials. In this study, the binding activity of L. fermentum PL9988 to the intestine was confirmed in another human intestinal cell line, HT-29, and mouse intestinal cells. The anti-inflammatory activity of L. fermentum PL9988 was examined via both in vitro and in vivo experiments. Lactobacillus fermentum PL9988 increased the amount of IL-10 and decreased the amount of TNF-α in HT-29 cells treated with LPS. Similar results were observed in an in vivo experiment with BALB/c mice fed L. fermentum PL9988. Thus, results from the previous study and this study demonstrate the beneficial characteristics of L. fermentum PL9988 as a good probiotic with anti-inflammatory, antioxidative, and immune-enhancing activities in addition to improving intestinal health.

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发酵乳杆菌PL9988与肠上皮细胞的结合及抗炎活性

发酵乳杆菌PL9988是从韩国长寿村80岁以上老人身上分离出来的。先前的研究结果表明，发酵乳杆菌PL9988作为一种益生菌具有多种特性，包括对酸和胆汁酸的抗性，免疫增强活性，对肠道细胞系Caco-2的粘附性，对各种肠道病原体的抑制作用，抗氧化活性以及对抗菌素的敏感性。本研究在另一种人肠道细胞系HT-29和小鼠肠道细胞中证实了L. fermentum PL9988与肠道的结合活性。通过体外和体内实验考察了发酵乳杆菌PL9988的抗炎活性。发酵乳杆菌PL9988能提高LPS处理HT-29细胞中IL-10的含量，降低TNF-α的含量。在BALB/c小鼠体内实验中也观察到类似的结果。因此，前期研究和本研究的结果表明，发酵乳杆菌PL9988是一种良好的益生菌，具有抗炎、抗氧化和增强免疫功能，并能改善肠道健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Probiotics and Prebiotics Nursing-Nutrition and Dietetics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： The International journal of Probiotics & Prebiotics publishes on online only in an open access format. This is a broad based international, interdisciplinary peer reviewed scientific journal for critical evaluation of research on prebiotics, probiotics and synbiotics. The major goal of this journal is to provide unbiased scientific data to students, researchers, healthcare providers, and the decision makers in the nutraceutical industry to help make informed choices about prebiotics, probiotics and synbiotics. To this end, the journal will publish original research articles and two types of review articles. First, we will publish a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, we will publish a critical evaluation of current human experimental data to help deliver products with medically proven use.