Dynamics of nuclear and cytoplasm maturation of bovine oocytes cultivated in vitro in medium supplemented with fulerol

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia Pub Date : 2022-12-01 DOI:10.1590/1678-4162-12808

Rogério Palhares, V. M. Prata, A. Leite, P. P. Nunes, A.M.P. Leo, M. Ladeira, E. Silva, Á. M. Borges

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Abstract

ABSTRACT The aim of this work was to evaluate, in vitro, the dynamics of nuclear and cytoplasmic maturation of bovine oocytes in traditional IVM medium (CT) and supplemented with fullerol (MF50), for 36 hours. The nuclear maturation of CT (n=300) and MF50 (n=270) every 6 hours, stained with Hoechst33342 and cytoplasmic, the mitochondrial distribution of CT (n=197) and MF50 (n=159) at every 12 hours, stained with Mitotracker Orange. At 6 hours, CT oocytes (19%) were in MI (metaphase I), while in MF50 they were in GV (germ vesicle) or GVB (GV breakeage), repeating at 12 hours. At 18 hours, 46.3% were matured in CT, and 20% in MF50. At 24 hours, 43.9% of maturation was observed in the MF50 group, and 63.8% in the CT. At 30 and 36 hours, the maturation pattern was stable, but with the onset of oocyte degeneration. There was a delay in cytoplasmic maturation with 36 hours (P<0.05) in MF50 (53.9% of mature gametes), compared to CT (69.8%). With immature cytoplasm, they were 10.4% and 31.7% for CT and MF50 (P<0.05), respectively. It was concluded that fullerol possibly interfered in the expansion of cumulus oophorus cells, as well as delayed the meiotic progression and cytoplasmic maturation.

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牛卵母细胞在添加黄甾醇的培养基中的核质和细胞质成熟动力学

本研究的目的是在体外评估牛卵母细胞在传统IVM培养基(CT)和补充富勒醇(MF50)中36小时的核和细胞质成熟动力学。CT (n=300)和MF50 (n=270)每6小时的核成熟，用Hoechst33342和细胞质染色;CT (n=197)和MF50 (n=159)每12小时的线粒体分布，用Mitotracker Orange染色。在6小时时，CT卵母细胞(19%)处于MI(中期I)，而在MF50时，它们处于GV(生殖囊泡)或GVB (GV破裂)，在12小时重复。18小时时，CT成熟46.3%，MF50成熟20%。24小时时，MF50组的成熟率为43.9%，CT组为63.8%。在30和36小时，成熟模式是稳定的，但开始卵母细胞变性。MF50(53.9%)的成熟配子比CT(69.8%)延迟36小时(P<0.05)。细胞质未成熟时，CT和MF50分别为10.4%和31.7% (P<0.05)。结果表明，富勒醇可能干扰了卵丘细胞的扩张，延缓了卵丘细胞减数分裂的进程和细胞质的成熟。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 农林科学-兽医学

CiteScore

0.80

自引率

25.00%

发文量

111

审稿时长

9-18 weeks

期刊介绍： Publica artigos originais de pesquisa sobre temas de medicina veterinária, zootecnia, tecnologia e inspeção de produtos de origem animal e áreas afins relacionadas com a produção animal. Atualmente a revista mantém 628 permutas (419 internacionais e 209 nacionais), sendo um verdadeiro suporte para o recebimento de periódicos pela Biblioteca da Escola. A partir de 1999, a Escola de Veterinária delegou à FEP MVZ Editora o encargo do gerenciamento e edição de todas suas publicações, inclusive do Arquivo, ficando somente com o apoio logístico (instalações, equipamentos, pessoal etc.). O apoio financeiro é exercido pelo CNPq/FINEP e pela própria FEP MVZ.