PRELIMINARY EXAMINATION OF THE EXOSKELETAL MICROBIOTA OF ARMADILLIDIUM VULGARE (THE COMMON PILL BUG)

IF 0.2 4区环境科学与生态学 Q4 Agricultural and Biological Sciences

Southwestern Naturalist Pub Date : 2023-04-12 DOI:10.1894/0038-4909-67.1.10

Sondos Alhajouj, D. Starkey

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引用次数: 0

Abstract

Abstract The purpose of this study was to preliminarily examine the breadth of microbial diversity on the pill bug (Armadillidium vulgare) exoskeleton. To address this question, we collected pill bugs at four time points (approximately 3 months apart) and analyzed their external microbiotas. Preliminary results suggest that each time point contained a unique microbiome, suggesting a rapid turnover of the exoskeletal microbiota. In total, we recovered 15 unique isolates, which we resolved into three phyla and nine families. There were only three instances in which we recovered a single isolate from multiple time points, and we recovered only a single isolate from all four time points. Among the phyla identified, sequence divergence ranged from a minimum of 6.2% (among isolates recognized within the Actinobacteria) to a maximum of ∼34% (between isolates recognized within the Actinobacteria vs. b-proteobacteria). Overall, the results of this study suggest that in order to gain a complete understanding of the exoskeletal microbiota of pill bugs, and perhaps other arthropods, researchers must examine a temporal scale. Resumen El propósito de este estudio fue examinar preliminarmente la amplitud de la diversidad microbiana en el exoesqueleto de la cochinilla (Armadillidium vulgare). Para abordar esta pregunta, se recolectaron cochinillas en cuatro temporadas (aproximadamente tres meses entre si) y se analizaron sus microbiotas externas. Los resultados preliminares sugieren que cada temporada contuvo un microbioma único, lo que sugiere una rápida renovación de la microbiota exoesquelética. En total, se recuperaron 15 aislamientos únicos, que se resolvieron en tres filos y nueve familias. Solo hubo tres instancias en las que se recuperó un solo aislamiento de múltiples temporadas y se recuperó solo un aislamiento de todas las cuatro temporadas. Entre los filos identificados, la divergencia de secuencias varió desde un mínimo del 6.2% (entre aislados reconocidos dentro de Actinobacteria) hasta un máximo de ∼34% (entre aislados reconocidos dentro de Actinobacteria frente a b-proteobacteria). En general, los resultados de este estudio sugieren que para obtener una comprensión completa de la microbiota exoesquelética de las cochinillas y quizás de otros artrópodos, se debe examinar a escala temporal.

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普通丸虫犰狳(armadillidium vulgare)外骨骼微生物群的初步研究

摘要这项研究的目的是初步研究犰狳（Armadillidium vulgare）外骨骼微生物多样性的规律。为了解决这个问题，我们在四个时间点（大约3个月前）收集了药虫，并分析了它们的外部微生物群。初步结果表明，每个时间点都包含一个独特的微生物组，表明外骨骼微生物群迅速转变。总共恢复了15个独特的孤立物，我们将其分解为三个门和九个家庭。只有三个例子，我们从多个时间点恢复了一个单一的孤立，我们从所有四个时间点恢复了一个单一的孤立。在已鉴定的门中，序列差异从最低6.2%（在放线菌内识别的隔离物中）到最高~34%（在放线菌内识别的隔离物与B-蛋白杆菌之间）。总体而言，这项研究的结果表明，为了全面了解球虫和其他节肢动物的外骨骼微生物群，研究人员必须检查一个时间尺度。摘要本研究的目的是初步研究胭脂鱼（Armadillidium vulgare）外骨骼微生物多样性的广度。为了解决这个问题，在四个季节（彼此之间约三个月）收集了小猪，并分析了它们的外部微生物群。初步结果表明，每个季节都包含一个独特的微生物群，这表明外骨骼微生物群迅速更新。共恢复了15次独特的隔离，分为三个方向和9个家庭。只有三次在多个赛季中恢复了一次隔离，在所有四个赛季中只恢复了一次隔离。在已确定的边缘中，序列差异从最低6.2%（在放线菌内识别的菌株之间）到最高34%（在放线菌内识别的菌株与B-蛋白杆菌之间）不等。总体而言，这项研究的结果表明，为了全面了解猪的外骨骼微生物群，也许还有其他节肢动物，必须在时间范围内进行检查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Southwestern Naturalist 环境科学-生态学

CiteScore

0.50

自引率

50.00%

发文量

审稿时长

18-36 weeks

期刊介绍： The Southwestern Naturalist (a publication of the Southwestern Association of Naturalists since 1953) is an international journal (published quarterly) that reports original and significant research in any field of natural history. This journal promotes the study of plants and animals (living and fossil) in the multinational region that includes the southwestern United States, Mexico, and Central America. Appropriate submission of manuscripts may come from studies conducted in the countries of focus or in regions outside this area that report significant findings relating to biota occurring in the southwestern United States, Mexico, and Central America. Publication is in English, and manuscripts may be feature articles or notes. Feature articles communicate results of completed scientific investigations, while notes are reserved for short communications (e.g., behavioral observations, range extensions, and other important findings that do not in themselves constitute a comprehensive study). All manuscripts (feature articles and notes) require an abstract in both English and Spanish.