Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila.

IF 0.8 4区农林科学 Q3 FISHERIES

Iranian Journal of Fisheries Sciences Pub Date : 2020-11-10 DOI:10.22092/IJFS.2020.122770

S.M.A. Ebnetorab, H. Ahari, S. Kakoolaki

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引用次数: 3

Abstract

Our study was focused on characterization of dominantBacillus bacteria isolated from the digestive tract of Oncorhynchus mykiss. After 8 weeks, the fish fed with probiotic-free diet were dissected under strictly controlled conditions. Fish intestines were eviscerated and its content diluted to 10−4 using Ringer solution. Two isolated bacteria were Gram-positive, catalase-positive, and oxidase-negative, based on the biochemical tests. A mixed of the two strains was the most efficient isolates determined inO. mykiss. Molecular characterization using PCR-Ribotyping was carried out using sequencing the 16S rRNA gene of both strains, which isolated two bacteria, Bacillus subtilis Ahari.H1 and B. licheniformis Ahari. H2. The isolated bacteria could tolerate 0.3% bile salt and non-hemolytic in nature. They also showed resistance to pH at 2.5 and 4 for acid test and 2.3 and 7 for gastric juice, pepsin and trypsin. It is concluded that the efficiency of the isolated probiotics can be candidates as probiotic in fish culture. It was unveiled that these strains can successfully diminish Aeromonas hydrophila pathogenicity in rainbow trout.

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虹鳟消化系统枯草芽孢杆菌和地衣芽孢杆菌的分离、生化和分子检测及其对嗜水气单胞菌的抑制作用。

本文研究了从密吻Oncorhynchus消化道分离的优势芽孢杆菌的特性。8周后，在严格控制的条件下对饲喂无益生菌饲料的鱼进行解剖。取出鱼肠，用林格液将其含量稀释至10−4。根据生化试验，两个分离的细菌革兰氏阳性，过氧化氢酶阳性，氧化酶阴性。两种菌株的混合是最有效的分离物。mykiss。采用PCR-Ribotyping方法对两株菌株进行分子鉴定，并对其16S rRNA基因进行测序，分离得到枯草芽孢杆菌Ahari。H1和地衣芽孢杆菌。H2。分离的细菌能耐受0.3%的胆盐，性质上无溶血性。它们对酸液、胃蛋白酶和胰蛋白酶的pH值分别为2.5和4，对胃液、胃蛋白酶和胰蛋白酶的pH值分别为2.3和7。结果表明，分离得到的益生菌可作为鱼类养殖益生菌的候选菌。结果表明，这些菌株能有效地降低虹鳟鱼嗜水气单胞菌的致病性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Fisheries Sciences FISHERIES-

CiteScore

2.30

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： 1- Living various species (contains animals and vegetal species) in various aquatic ecosystems. 2- Health and diseases of aquatic species. 3- Determining the stocks and specific time and location for catching and reliable exploitation for sustainable development. 4- Methods of propagation and culture of high value aquatic resources. 5- Aquatic stock assessment and the methods of restocking the high value species and suggestion for rate, areas and the time for releasing fish and other aquatic organisms fries. 6- Pollutant agents and their effects to the environments of aquatic species. 7- Feed and feeding in aquatic organisms. 8- Fish processing and producing new products. 9- The economic and social aspects of fisheries.