Dissecting Flux Balances to Measure Energetic Costs in Cell Biology: Techniques and Challenges

IF 14.3 1区物理与天体物理 Q1 PHYSICS, CONDENSED MATTER

Annual Review of Condensed Matter Physics Pub Date : 2022-05-02 DOI:10.1146/annurev-conmatphys-031620-105251

Easun Arunachalam, W. Ireland, Xingbo Yang, D. Needleman

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引用次数: 1

Abstract

Life is a nonequilibrium phenomenon: Metabolism provides a continuous supply of energy that drives nearly all cellular processes. However, very little is known about how much energy different cellular processes use, i.e., their energetic costs. The most direct experimental measurements of these costs involve modulating the activity of cellular processes and determining the resulting changes in energetic fluxes. In this review, we present a flux balance framework to aid in the design and interpretation of such experiments and discuss the challenges associated with measuring the relevant metabolic fluxes. We then describe selected techniques that enable measurement of these fluxes. Finally, we review prior experimental and theoretical work that has employed techniques from biochemistry and nonequilibrium physics to determine the energetic costs of cellular processes. Expected final online publication date for the Annual Review of Condensed Matter Physics, Volume 14 is March 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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细胞生物学中解析通量平衡以测量能量成本：技术和挑战

生命是一种非平衡现象：新陈代谢提供了持续的能量供应，几乎驱动了所有的细胞过程。然而，对于不同的细胞过程使用多少能量，即它们的能量成本，我们知之甚少。对这些成本最直接的实验测量涉及调节细胞过程的活性，并确定由此产生的能量通量变化。在这篇综述中，我们提出了一个通量平衡框架，以帮助设计和解释这些实验，并讨论了与测量相关代谢通量相关的挑战。然后，我们描述了能够测量这些通量的选定技术。最后，我们回顾了先前的实验和理论工作，这些工作采用了生物化学和非平衡物理学的技术来确定细胞过程的能量成本。《凝聚态物理学年度评论》第14卷预计最终在线出版日期为2023年3月。请参阅http://www.annualreviews.org/page/journal/pubdates用于修订估算。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annual Review of Condensed Matter Physics PHYSICS, CONDENSED MATTER-

CiteScore

47.40

自引率

0.90%

发文量

期刊介绍： Since its inception in 2010, the Annual Review of Condensed Matter Physics has been chronicling significant advancements in the field and its related subjects. By highlighting recent developments and offering critical evaluations, the journal actively contributes to the ongoing discourse in condensed matter physics. The latest volume of the journal has transitioned from gated access to open access, facilitated by Annual Reviews' Subscribe to Open initiative. Under this program, all articles are now published under a CC BY license, ensuring broader accessibility and dissemination of knowledge.