Correction of the Chlorophyll a Fluorescence Quenching in the Sea Upper Mixed Layer: Development of the Algorithm

IF 0.7 Q4 OCEANOGRAPHY
N. Moiseeva, T. Churilova, T. Efimova, D. Matorin
{"title":"Correction of the Chlorophyll a Fluorescence Quenching in the Sea Upper Mixed Layer: Development of the Algorithm","authors":"N. Moiseeva, T. Churilova, T. Efimova, D. Matorin","doi":"10.22449/0233-7584-2020-1-66-74","DOIUrl":null,"url":null,"abstract":"Purpose. Data from the immersion fluorescence sensors of the CTD complex and BioArgo floats are widely used for studying spatial and temporal variability of the chlorophyll a concentration on large scales. In these devices the fluorescence sensors are not equipped with a dark chamber; as a result, a part of the reaction centers of photosystem 2 is closed due to the sunlight effect. It is manifested in decrease of the measured fluorescence intensity in the upper mixed layer due to fluorescence quenching, while the chlorophyll a concentration can remain unchanged. The purpose of the work is to develop the algorithm for correcting the fluorescence quenching. Methods and Results. It is shown that photosynthetically available radiation decreases with depth within the upper mixed layer by almost an order, and the chlorophyll a fluorescence measured by the immersion sensor (without a dark chamber), increases with depth in this layer. Relationship between light intensity and share of open reaction centers of photosystem 2 in cell of microalgae was revealed. The relationship is described by the exponential function. The share of open centers effects on the degree of fluorescence intensity decreasing and therefore on fluorescence quenching induced by light. Conclusions. The universal algorithm for correcting fluorescence quenching in the upper mixed layer is proposed. Due to its correction, almost uniform fluorescence distribution is obtained in the upper mixed layer which is in a good consistence with the results of chlorophyll a concentration measurements in situ.","PeriodicalId":43550,"journal":{"name":"Physical Oceanography","volume":" ","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2020-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physical Oceanography","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22449/0233-7584-2020-1-66-74","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"OCEANOGRAPHY","Score":null,"Total":0}
引用次数: 5

Abstract

Purpose. Data from the immersion fluorescence sensors of the CTD complex and BioArgo floats are widely used for studying spatial and temporal variability of the chlorophyll a concentration on large scales. In these devices the fluorescence sensors are not equipped with a dark chamber; as a result, a part of the reaction centers of photosystem 2 is closed due to the sunlight effect. It is manifested in decrease of the measured fluorescence intensity in the upper mixed layer due to fluorescence quenching, while the chlorophyll a concentration can remain unchanged. The purpose of the work is to develop the algorithm for correcting the fluorescence quenching. Methods and Results. It is shown that photosynthetically available radiation decreases with depth within the upper mixed layer by almost an order, and the chlorophyll a fluorescence measured by the immersion sensor (without a dark chamber), increases with depth in this layer. Relationship between light intensity and share of open reaction centers of photosystem 2 in cell of microalgae was revealed. The relationship is described by the exponential function. The share of open centers effects on the degree of fluorescence intensity decreasing and therefore on fluorescence quenching induced by light. Conclusions. The universal algorithm for correcting fluorescence quenching in the upper mixed layer is proposed. Due to its correction, almost uniform fluorescence distribution is obtained in the upper mixed layer which is in a good consistence with the results of chlorophyll a concentration measurements in situ.
海面上层混合层叶绿素a荧光猝灭校正算法的发展
目的。CTD复合物和BioArgo浮标的浸没荧光传感器数据被广泛用于大尺度上叶绿素a浓度的时空变化研究。在这些装置中,荧光传感器没有配备暗室;结果,光系统2的部分反应中心由于阳光效应而关闭。表现为由于荧光猝灭,上层混合层测得的荧光强度降低,而叶绿素a浓度可以保持不变。本工作的目的是开发校正荧光猝灭的算法。方法与结果。结果表明,在上层混合层中,光合有效辐射随深度的增加而减少近一个数量级,而浸没传感器(无暗室)测量的叶绿素a荧光随深度的增加而增加。揭示了光强与微藻细胞光系统2开放反应中心比例的关系。这种关系用指数函数来描述。开中心的比例影响荧光强度的降低程度,从而影响光诱导的荧光猝灭。结论。提出了校正上层混合层荧光猝灭的通用算法。由于校正,上层混合层的荧光分布基本均匀,与原位测量的叶绿素a浓度结果吻合较好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Physical Oceanography
Physical Oceanography OCEANOGRAPHY-
CiteScore
1.80
自引率
25.00%
发文量
8
审稿时长
24 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信