Changes in the functional activity of the hypothalamic-pituitaryadrenal axis in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism

Cardiometry Pub Date : 2023-05-04 DOI:10.18137/cardiometry.2023.27.4046

O. Kit, E. Frantsiyants, V. Bandovkina, I. Kaplieva, E. Surikova, I. Neskubina, N. Cheryarina, L. Trepitaki, Y. Pogorelova, I. Goroshinskaya, G. Zhukova, A. Arakelova, L. Vaschenko, V. Kovalenko, O. V. Bykadorova, A.S. Kuligina

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Abstract

The hypothalamic-pituitary-adrenal axis (HPA) makes the body’s response to various stress factors, interacting with the thyroid and gonadal axis. The aim of our work was to study the effect by induced hypothyroidism on changes in the functioning of the HPA axis in rats with Guerin’s carcinoma of both sexes. Materials and methods. The experiment was performed in white outbred rats of both sexes. Hypothyroidism was induced in animals by medication for 30 days with Mercazolil, and then Guerin’s carcinoma was transplanted (the main group). The reference groups covered animals with the independent growth of Guerin’s carcinoma and with independent hypothyroidism, as well as intact animals (the normal conditions). On day 18 of the tumor growth, the animals were sacrificed, and, using standard ELISA kits, in the serum and the homogenates of the adrenal glands, the tumor and the perifocal zone, the level of cortisol was determined; in the homogenates of the hypothalamus we measured the concentration of CT-releasing hormone (CRH) and in the pituitary gland the concentration of ACTH. Results. Induced hypothyroidism both in males and females caused adrenal insufficiency. The growth of Guerin’s carcinoma increased the level of CT-releasing hormone in males by 9.6 times and cortisol in the adrenal glands in females and males by 1.3 times and 2.3 times (p<0.05), respectively. In the main group females and males we revealed a decrease in the level of CT-releasing hormone of the hypothalamus by an average of 1.4 times, ACTH in the pituitary gland by 1.5-1.8 times, but an increase in the cortisol concentration in blood and the adrenal glands only in males by an average of 1.5 times (p˂0.05). Only in males of the main group in the tumor and its perifocal zone, the content of cortisol increased by 5.9 times and 1.6 times (р˂0.05), respectively. Conclusion. The HPA axis has a gender-specific response to the growth of a malignant tumor, both in its independent growth variant and against the background of hypothyroidism, which in its independent variant causes adrenal insufficiency.

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甲状腺功能减退背景下两性Guerin癌大鼠下丘脑-垂体-肾上腺轴功能活动的变化

下丘脑-垂体-肾上腺轴(HPA)与甲状腺和性腺轴相互作用，使身体对各种应激因素作出反应。我们的工作目的是研究诱导甲状腺功能减退对两性Guerin癌大鼠HPA轴功能变化的影响。材料和方法。该实验是在雌雄异种的白色大鼠中进行的。用美卡唑利给药30 d诱导动物甲状腺功能减退，然后移植葛兰癌(主要组)。参照组包括独立生长的Guerin癌和独立甲状腺功能减退的动物，以及正常情况下的完整动物。肿瘤生长第18天，处死动物，采用标准ELISA试剂盒检测血清及肾上腺、肿瘤及焦周区匀浆中的皮质醇水平;在下丘脑匀浆中，我们测量了ct释放激素(CRH)的浓度，在脑垂体中测量了ACTH的浓度。结果。诱发性甲状腺功能减退症在男性和女性均引起肾上腺功能不全。Guerin癌的生长使男性ct释放激素升高9.6倍，女性肾上腺皮质醇升高1.3倍，男性肾上腺皮质醇升高2.3倍(p<0.05)。在主组中，我们发现女性和男性下丘脑的ct释放激素水平平均下降了1.4倍，垂体ACTH下降了1.5-1.8倍，但血液和肾上腺中的皮质醇浓度仅在男性中平均增加了1.5倍(p小于0.05)。仅在肿瘤及其焦周区主组男性中，皮质醇含量分别增加了5.9倍和1.6倍(p < 0.05)。结论。HPA轴对恶性肿瘤的生长具有性别特异性反应，无论是在其独立生长变异中，还是在甲状腺功能减退的背景下，其独立变异导致肾上腺功能不全。

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来源期刊

Cardiometry MEDICAL LABORATORY TECHNOLOGY-

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审稿时长

6 weeks

期刊介绍： Cardiometry is an open access biannual electronic journal founded in 2012. It refers to medicine, particularly to cardiology, as well as oncocardiology and allied science of biophysics and medical equipment engineering. We publish mainly high quality original articles, reports, case reports, reviews and lectures in the field of the theory of cardiovascular system functioning, principles of cardiometry, its diagnostic methods, cardiovascular system therapy from the aspect of cardiometry, system and particular approaches to maintaining health, engineering peculiarities in cardiometry developing. The interdisciplinary areas of the journal are: hemodynamics, biophysics, biochemistry, metrology. The target audience of our Journal covers healthcare providers including cardiologists and general practitioners, bioengineers, biophysics, medical equipment, especially cardiology diagnostics device, developers, educators, nurses, healthcare decision-makers, people with cardiovascular diseases, cardiology and engineering universities and schools, state and private clinics. Cardiometry is aimed to provide a wide forum for exchange of information and public discussion on above scientific issues for the mentioned experts.