A rapid sampling technique for isolating highly productive lipid-rich algae strains from environmental samples

IF 14.4 Q1 ENERGY & FUELS
Suting Huang, Jo L. Goh, H. Ahmadzadeh, M. Murry
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引用次数: 10

Abstract

Strain selection and isolation of lipid-rich microalgae are among the two most important steps for screening isolates with maximum biofuel productivity. In this work, we introduce a novel direct sampling technique that allows native strains to be selected for rapid growth under defined conditions followed by direct selection of product-rich species, two desirable characteristics of algae for mass culture. This sampling strategy directly selects the lipid-rich strains visualized under an inverted fluorescence microscope using an X-Y-Z micromanipulator. The enrichment step can be manipulated to select for strains with specific technological applications. Direct sampling of lipid-rich cells avoids the tedious task of screening isolates while using relatively inexpensive equipment.
从环境样品中分离高产富脂藻类菌株的快速取样技术
选择菌株和分离富含脂质的微藻是筛选具有最大生物燃料生产力的分离物的两个最重要的步骤。在这项工作中,我们介绍了一种新的直接采样技术,该技术允许选择本地菌株在特定条件下快速生长,然后直接选择富含产品的物种,这是大规模培养藻类的两个理想特征。这种采样策略使用X-Y-Z微操作器直接选择在倒置荧光显微镜下观察到的富含脂质的菌株。富集步骤可以进行操作以选择具有特定技术应用的菌株。富含脂质的细胞的直接采样避免了筛选分离物的繁琐任务,同时使用相对便宜的设备。
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来源期刊
CiteScore
22.10
自引率
1.50%
发文量
15
审稿时长
8 weeks
期刊介绍: Biofuel Research Journal (BRJ) is a leading, peer-reviewed academic journal that focuses on high-quality research in the field of biofuels, bioproducts, and biomass-derived materials and technologies. The journal's primary goal is to contribute to the advancement of knowledge and understanding in the areas of sustainable energy solutions, environmental protection, and the circular economy. BRJ accepts various types of articles, including original research papers, review papers, case studies, short communications, and hypotheses. The specific areas covered by the journal include Biofuels and Bioproducts, Biomass Valorization, Biomass-Derived Materials for Energy and Storage Systems, Techno-Economic and Environmental Assessments, Climate Change and Sustainability, and Biofuels and Bioproducts in Circular Economy, among others. BRJ actively encourages interdisciplinary collaborations among researchers, engineers, scientists, policymakers, and industry experts to facilitate the adoption of sustainable energy solutions and promote a greener future. The journal maintains rigorous standards of peer review and editorial integrity to ensure that only impactful and high-quality research is published. Currently, BRJ is indexed by several prominent databases such as Web of Science, CAS Databases, Directory of Open Access Journals, Scimago Journal Rank, Scopus, Google Scholar, Elektronische Zeitschriftenbibliothek EZB, et al.
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