Effect of Crocin on miRNA-15a expression in EBV infected transformed B cell

Abstract

Despite in and cancer is still the main cause of death worldwide. Crocin, the major carotenoid in saffron, exhibits potent anti-tumor effects in breast, pancreas, and gastric tumors. However, the anti-lymphoma effects of crocin are still unclear, especially in B cell lymphomas. The present study aimed to evaluate the cytotoxic effects of crocin on EBV-infected transformed B cell. an (EBV) The crocin (0.2-200 3 Cell viability and were using MTT, and flow cytometry techniques respectively. Also, miRNA-15a genes expression were determined by real-time PCR. of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05). Conclusions: The results suggested that crocin can be considered for preclinical and also clinical testing in EBV-associated B cell lymphoma treatment. The results showed that due to the effect of crocin in reducing the survival and increasing apoptosis of lymphoma cells and also due to the lack of change in the expression of miRNA-15a, it can be considered as a therapeutic candidate in the treatment of EBV-associated B cell lymphoma.