Estimation of in vitro digestibility and fermentation of elephant grass by near infrared spectroscopy

IF 1.1 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY
Joadil G. Abreu, Víctor M. Fernández-Cabanás, Janderson A. Rodrigues, Matheus Abreu, Eduardo A. Ferreira, Wender M. Peixoto
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引用次数: 1

Abstract

The methods used to evaluate kinetic parameters of ruminant feeds can be in situ and in vitro. For both methods, it is necessary to maintain cannulated animals in order to collect the inoculum from the rumen, which has been under strong pressure from society, in terms of animal welfare policies, to avoid this type of practices. This work aimed to evaluate the estimation of in vitro digestibility and fermentation of elephant grass forage by near infrared reflectance spectroscopy (NIRS), as a fast and noninvasive alternative to the in vitro method. The experimental design was in randomized blocks with three repetitions. The treatments were arranged in a split-plot scheme, with 13 genotypes in the plot and 15 regrowth ages in the subplot. Crude protein (CP) and neutral detergent fiber (NDF) contents; in vitro dry matter (IVDMD) and NDF (IVNDFD) digestibilities; and total volume (Vt), gas production rate (μ) and half-life conventional analyses were determined for pre-dried elephant grass forage samples. For the evaluation of NIRS models performance, coefficient of determination (R2cv) and standard error of cross validation (SECV) were used. As the regrowth age advanced, there was a linear increase in the NDF content and a linear reduction in the IVDMD, IVNDFD and Vt values. The NIRS estimates proved to be adequate for IVDMD (R2cv = .95), IVNDFD (R2cv = .85), Vt (R2cv = .81) and gas production at 48 (R2cv = .82), 72 (R2cv = .85) and 96 h (R2cv = .84), confirming as an alternative to the in vitro methods that dispense with the use of cannulated animals.

近红外光谱法测定象草的体外消化率和发酵率
反刍动物饲料动力学参数的评价方法可分为原位和体外两种。对于这两种方法,都需要保持接种动物,以便从瘤胃中收集接种物,这在社会上一直受到很大的压力,从动物福利政策上来说,避免这种做法。本研究旨在探讨近红外反射光谱法(NIRS)对象草饲料体外消化率和发酵率的快速、无创评价方法。实验设计为随机分组,重复3次。处理按分块处理,分块处理13个基因型,分块处理15个再生年龄。粗蛋白质(CP)和中性洗涤纤维(NDF)含量;体外干物质(IVDMD)和NDF (IVNDFD)消化率;对预干燥的象草草料样品进行了总体积(Vt)、产气速率(μ)和半衰期常规分析。为了评价NIRS模型的性能,使用了决定系数(R2cv)和交叉验证标准误差(SECV)。随着再生龄期的延长,NDF含量呈线性增加,IVDMD、IVNDFD和Vt值呈线性降低。NIRS估计证明适合于IVDMD (R2cv = 0.95)、IVNDFD (R2cv = 0.85)、Vt (R2cv = 0.81)和48 (R2cv = 0.82)、72 (R2cv = 0.85)和96 h (R2cv = 0.84)的产气量,证实可以替代体外方法,无需使用插管动物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Grassland Science
Grassland Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
2.70
自引率
7.70%
发文量
38
审稿时长
>12 weeks
期刊介绍: Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields: grassland environment, landscape, ecology and systems analysis; pasture and lawn establishment, management and cultivation; grassland utilization, animal management, behavior, nutrition and production; forage conservation, processing, storage, utilization and nutritive value; physiology, morphology, pathology and entomology of plants; breeding and genetics; physicochemical property of soil, soil animals and microorganisms and plant nutrition; economics in grassland systems.
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