Effect of Isothiocyanate Therapy on Apoptosis of Trophoblast Cells in Pregnant Rats Hyperglycemia Atmosphere

Berkala Kedokteran Pub Date : 2023-02-28 DOI:10.20527/jbk.v19i1.15709

H. Gondo, Elizabteh Haryanti

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Abstract

Abstract: One of the factors that influence the complications of pregnancy and childbirth is diabetes mellitus. In this study, it was intended to create experimental diabetic conditions in experimental white rats with the administration of aloksan for 5 days. A total of 30 mice were checked for sugar levels on day 7 after inducing alloxane to see that the mice were already in a hyperglycemic state. Then divided into 6 groups, each group there are 5 white pregnant rats. Group 1 was the negative group (without being given aloksan), group 2 was positive group (given aloksan as much as 150 mg / day / kg BB), groups 3, 4, 5 and 6 were given Aloksan 150mg / day / kg and each was given simultaneously isothiocyanates with a dose of 0.1; 0,2; 0.4 and 0.8 mg/day/kg BB. DNA fragmentation is one of the characteristics of apoptosis. In this study DNA fragmentation was examined by the TUNEL method (Terminal deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling). The average number of trophoblast cells in white pregnant rats hyperglycemia that experienced apoptosis decreased as the dose of isotiocyanate therapy increased in rats that had been induced by alloxane. And the best dose is at a dose of 0.8 mg / day / kg BB.

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异硫氰酸酯治疗对妊娠高血糖大鼠滋养细胞凋亡的影响

摘要:糖尿病是影响妊娠和分娩并发症的因素之一。在本研究中，目的是在给药5天的实验白鼠中建立实验性糖尿病状态。共30只小鼠在诱导四氧烷后的第7天检查血糖水平，发现小鼠已经处于高血糖状态。然后分为6组，每组5只白孕大鼠。1组为阴性组(未给予阿洛克山酯)，2组为阳性组(给予阿洛克山酯150mg / d / kg BB)， 3、4、5、6组给予阿洛克山酯150mg / d / kg，同时给予异硫氰酸酯，剂量为0.1;0, 2;0.4和0.8毫克/天/千克BB。DNA断裂是细胞凋亡的特征之一。本研究采用末端脱氧核苷酸转移酶介导的dUTP Nick末端标记(TUNEL)方法检测DNA片段。四氧烷诱导的白孕高血糖大鼠滋养细胞平均凋亡数随着异氰酸盐剂量的增加而减少。最佳剂量为0.8 mg / d / kg BB。

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Berkala Kedokteran

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24 weeks