Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2022-09-30 DOI:10.4995/wrs.2022.17042

A. Fadl, K. El-Shahat, E. Abdelnaby

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引用次数: 1

Abstract

Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabbit bucks. Semen samples were diluted with a tris-citrate-glucose (TCG) extender supplemented with ALA (0, 50, 75 and 100 μmol). Then, extended rabbit semen was cooled at 5°C and cryopreserved in liquid nitrogen. After thawing, spermatozoal quality parameters (individual motility %, viability %, osmotic resistance %, and acrosome integrity %), antioxidant activity (SOD, CAT, and GSH activities), lipid peroxidation (malondialdehyde) and fertility (conception and kindling rates) were evaluated. Results revealed that supplementation of rabbit semen extender with 50 μmol ALA significantly (P<0.05) increased spermatozoal characteristics including motility (56.54%), viability (60.01%), acrosome status (72.66%) and membrane integrity (59.13%). The activity of semen antioxidant enzymes (SOD, CAT, and GSH) showed a significant improvement with a marked decrease in lipid peroxidation. Moreover, the conception (73.30%) and kindling (70.00%) rates were significantly (P<0.05) higher in does inseminated with thawed semen treated with 50 μmol ALA in comparison with other concentrations (0, 75 and 100 μmol). In summary, supplementation of rabbit semen extender with 50 μmol ALA improved motility, viability, membrane integrity, acrosome integrity, antioxidant enzymes activity and fertility of post-thaw rabbit spermatozoa. Our findings suggested that higher concentrations of ALA are detrimental to post-thaw characteristics of New Zealand white rabbit buck spermatozoa. To achieve better results, the semen freezing extender should be supplemented with ALA at lower concentrations, especially 50 μmol.

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α-亚麻酸对新西兰大白鼠冻融精液活力、抗氧化活性和生育力的影响

冷冻和解冻过程会产生和积累高浓度的活性氧，这对精子的活力和生育能力不利。因此，在冷冻稀释剂中补充外源抗氧化剂是至关重要的。本研究的目的是首次研究添加α-亚麻酸（ALA）的精液稀释剂是否可以改善解冻后兔精子的活力、活力、膜完整性、抗氧化状态和生育能力。精液是从15只新西兰大白兔中收集和汇集而成的。用补充有ALA（0、50、75和100μmol）的柠檬酸三酯-葡萄糖（TCG）扩展器稀释精液样品。然后，将延长的兔精液在5°C下冷却，并在液氮中冷冻保存。解冻后，评估精子质量参数（个体活力%、活力%、渗透阻力%和顶体完整性%）、抗氧化活性（SOD、CAT和GSH活性）、脂质过氧化（丙二醛）和生育能力（受孕率和点燃率）。结果表明，添加50μmol ALA能显著提高精子活力（56.54%）、活力（60.01%）、顶体状态（72.66%）和膜完整性（59.13%），并能显著提高精液中抗氧化酶（SOD、CAT和GSH）的活性。与其他浓度（0、75和100μmol）相比，用50μmol ALA处理的解冻精液受精的受精卵的受孕率（73.30%）和点燃率（70.00%）显著（P<0.05）。总之，添加50μmol ALA的兔精液扩展器改善了解冻后兔精子的活力、活力、膜完整性、顶体完整性、抗氧化酶活性和生育能力。我们的研究结果表明，较高浓度的ALA对新西兰大白兔雄鹿精子解冻后的特性有害。为了获得更好的效果，精液冷冻延长剂应补充较低浓度的ALA，特别是50μmol。

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.