Association of sex hormone-binding globulin gene polymorphism and sex hormone-binding globulin levels in postmenopausal women in relation to type 2 diabetes mellitus – A case–Control study

IF 1 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Priyadharshini Alva, NSuchetha Kumari, A. Bhandary, Lakshmi Manjeera, Neetha Nandan
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引用次数: 0

Abstract

Background: In women, menopause constitutes the end of a fertile life as a result of ovarian aging. It is characterized by a significant decrease in estrogen concentration along with the change in the body weight, altered distribution of adipose tissue, less energy expenditure as well as modification in insulin secretion, and insulin sensitivity resulting in the development of type 2 diabetes mellitus (T2DM). The low circulating concentration of sex hormone-binding globulin (SHBG) contributes to the increased risk of type 2 diabetes in postmenopausal women. Methods: The present study was conducted at Justice K. S. Hegde Charitable Hospital, Deralakatte, Mangaluru. This case–control study was performed on 400 postmenopausal women with and without T2DM, aged 45–65 years, recruited based on the eligibility criteria. Five milliliters of fasting blood were collected, centrifuged, and stored at −80°C until analysis. Genomic DNA was isolated from the whole blood using a standard protocol. DNA was amplified using the polymerase chain reaction. Amplified products were sequenced using the Sanger sequencing method. Statistical analyses were performed using the SPSS software version 26. Results: Based on the SHBG single-nucleotide polymorphisms (SNP) rs1799941, the circulating levels of SHBG showed a significant difference between the genotype. Conclusions: The major allele of rs1799941 has shown reduced the levels of circulating SHBG with an increased risk of T2DM. Hyperglycemia along its association with endogenous hormone and SNP in the SHBG gene may contribute to the diabetic risk in the postmenopausal women.
绝经后妇女性激素结合球蛋白基因多态性和性激素结合球蛋白水平与2型糖尿病的关系——一项病例对照研究
背景:在女性中,由于卵巢老化,更年期是生育期的结束。其特点是雌激素浓度随体重变化而显著降低,脂肪组织分布改变,能量消耗减少,胰岛素分泌改变,胰岛素敏感性改变,导致2型糖尿病(T2DM)的发生。低循环浓度的性激素结合球蛋白(SHBG)有助于增加绝经后妇女患2型糖尿病的风险。方法:本研究在Mangaluru Deralakatte Justice K. S. Hegde慈善医院进行。本病例对照研究纳入了400名绝经后伴有或不伴有2型糖尿病的妇女,年龄45-65岁,根据入选标准招募。收集5毫升空腹血,离心,保存在- 80°C以待分析。基因组DNA是用标准程序从全血中分离出来的。用聚合酶链反应扩增DNA。扩增产物采用Sanger测序法测序。采用SPSS软件26进行统计分析。结果:基于SHBG单核苷酸多态性(SNP) rs1799941, SHBG的循环水平在基因型之间存在显著差异。结论:rs1799941的主要等位基因显示,循环SHBG水平降低,T2DM风险增加。高血糖及其与内源性激素和SHBG基因SNP的关联可能会增加绝经后妇女患糖尿病的风险。
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来源期刊
Biomedical and Biotechnology Research Journal
Biomedical and Biotechnology Research Journal Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
2.20
自引率
42.90%
发文量
24
审稿时长
11 weeks
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