Natural infection of squash fruits (Cucurbita pepo) by Zucchini Yellow Mosaic potyvirus (ZYMV) in Alexandria governorate

Abstract

An isolate of zucchini yellow mosaic virus (ZYMV) was obtained from naturally infected squash fruits were grown in Abees region, Alexandria governorate. Disease symptoms were Showing mosaic, yellowing and blistering and absis symptoms. The identifi cation was based on the symptoms developed on diagnostic hosts and serological reactions with antisera to cucumber mosaic cucumovirus (CMV), watermelon mosaic potyvirus 2 (WMV-2) and ZYMV. Squash fruit isolate of ZYMV was transmitted by Aphis gossypii, aphis neri and Myzus persicae in non-persistent manner. The virus was purifi ed by ultra-centrifugation and PEG. The purifi ed virus had an ultraviolet absorption spectrum typical of a nucleoprotein with A260/280 and A280/260 being 1.1