Diagnostic value of flow cytometry in detecting human papillomavirus E6/E7 mRNA in cervical lesions

中国综合临床 Pub Date : 2019-11-01 DOI:10.3760/CMA.J.ISSN.1008-6315.2019.06.003

Hui-min Guo, Lei Zhu, Jingbo Gao, L. Bai, Zhiyong Sun, Jinli Zhang, Bo Yang, C. Hao

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Abstract

Objective To explore the diagnostic value of flow cytometry in detecting HPV E6/E7 mRNA of human papilloma virus (HPV) in the diagnosis of cervical lesions. Methods From January 2017 to September 2018, 119 women with suspected cervical lesions in the department of gynecology and obstetrics of Shanxi Maternal and Child Health Hospital were selected.Flow cytometry was used to detect HPV E6 / E7 mRNA in cervical exfoliated cells of women, and the DNA of HPV was detected by the method of hybrid capture 2 (HC2). Results 31.09% (37/119) HPV E6/E7 mRNA and 57.14% (68 / 119) HPV DNA were positive in 119 cases.The positive rate of HPV E6/E7 mRNA in cervical intraepithelial neoplasia (CIN)2+ group was 77.78% (28/36), which was statistically significant compared with 20.00% (4/20) in CIN1 group (χ2=15.246, P<0.01), and was statistically significant compared with 7.94% (5/63) in nilm group (χ2=50.286, P<0.01). In nilm group, HPV E6 / E7 mRNA positive rate was 7.94% (5/63) and HPV DNA positive rate was 30.16% (19 / 63), which was statistically significant (χ2=10.088, P=0.001). In cin1 group, HPV E6/ E7 mRNA positive rate was 20.00% (4 / 20) and HPV DNA positive rate was 85.00% (17/20), which was statistically significant (χ2=14.436, P<0.001). The sensitivity, specificity, positive predictive value and negative predictive value of HPV E6 / E7 mRNA detected by flow cytometry were 75.00%, 89.16%, 72.72% and 90.24%, respectively.The sensitivity, specificity, positive predictive value and negative predictive value ofthe detection of HPV DNA by HC2 were 88.89%, 56.63%, 47.06% and 92.16%, respectively. Conclusion The specificity and positive predictive value of HPV E6 / E7 mRNA detected by flow cytometry were better than those detected by HC2.The detection of HPV E6 / E7 mRNA has potential clinical value in cervical precancerous lesions screening. Key words: Cervical intraepithelial neoplasia; Flow cytometry; Papillomavirus family; Papillomavirus E7 Proteins

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流式细胞术检测宫颈病变中人乳头瘤病毒E6/E7 mRNA的诊断价值

目的探讨流式细胞术检测人乳头瘤病毒(HPV) E6/E7 mRNA对宫颈病变的诊断价值。方法选取2017年1月~ 2018年9月山西省妇幼保健院妇产科疑似宫颈病变患者119例。采用流式细胞术检测女性宫颈脱落细胞中HPV E6 / E7 mRNA，采用杂交捕获2 (HC2)法检测HPV DNA。结果119例患者中HPV E6/E7 mRNA和HPV DNA阳性率分别为31.09%(37/119)和57.14%(68 /119)。宫颈上皮内瘤变(CIN)2+组HPV E6/E7 mRNA阳性率为77.78%(28/36)，与CIN1组的20.00%(4/20)相比，差异有统计学意义(χ2=15.246, P<0.01);与nilm组的7.94%(5/63)相比，差异有统计学意义(χ2=50.286, P<0.01)。nilm组HPV E6 / E7 mRNA阳性率为7.94% (5/63)，HPV DNA阳性率为30.16%(19 /63)，差异有统计学意义(χ2=10.088, P=0.001)。cin1组HPV E6/ E7 mRNA阳性率为20.00% (4 /20)，HPV DNA阳性率为85.00%(17/20)，差异有统计学意义(χ2=14.436, P<0.001)。流式细胞术检测HPV E6 / E7 mRNA的敏感性为75.00%，特异性为89.16%，阳性预测值为72.72%，阴性预测值为90.24%。HC2检测HPV DNA的敏感性为88.89%，特异性为56.63%，阳性预测值为47.06%，阴性预测值为92.16%。结论流式细胞术检测HPV E6 / E7 mRNA的特异性和阳性预测值均优于HC2检测。HPV E6 / E7 mRNA的检测在宫颈癌前病变筛查中具有潜在的临床价值。关键词:宫颈上皮内瘤变;流式细胞术;乳头状瘤病毒的家庭;乳头瘤病毒E7蛋白

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来源期刊

中国综合临床

CiteScore

0.10

自引率

0.00%

发文量

16855

期刊介绍： Clinical Medicine of China is an academic journal organized by the Chinese Medical Association (CMA), which mainly publishes original research papers, reviews and commentaries in the field. Clinical Medicine of China is a source journal of Peking University (2000 and 2004 editions), a core journal of Chinese science and technology, an academic journal of RCCSE China Core (Extended Edition), and has been published in Chemical Abstracts of the United States (CA), Abstracts Journal of Russia (AJ), Chinese Core Journals (Selection) Database, Chinese Science and Technology Materials Directory, Wanfang Database, China Academic Journal Database, JST Japan Science and Technology Agency Database (Japanese) (2018) and other databases.