Enriched rehabilitation training can improve the cognitive dysfunction of chronic cerebral hypoperfusion rats

Neuroscience informatics Pub Date : 2022-06-01 DOI:10.1016/j.neuri.2022.100050

Yixin Zhao , Wenping Gu , Hao Zhang , Jingyan Sun , Wenjin Ma , Yumeng Dong , Jingjing Nie

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Abstract

Objective: To investigate the molecular mechanism of enriched rehabilitation training in improving cognitive dysfunction of ischemic stroke.

Material and method: We established a rat model of cerebral ischemia with cognitive impairment and then carried out them to 4 groups according to different treatments. The all groups were performed Water maze training and cognitive function assessment. Western blot and Immunofluorescence were used to analyze the expression of synaptic plasticity-related proteins, regulatory proteins, and PKMζ in hippocampus. The patch clamp technique was used to record the field potential changes in the hippocampus and analyze the induction of LTP, and to observe the changes in field potential and LTP after the addition of PKMζ inhibitors.

Results: The results showed that enriched rehabilitation training improved cognitive function in stroke rats, increased the PKMζ and SYN protein levels, and enhanced LTP in the ischemia hippocampus. The enhanced LTP for the rats after enriched rehabilitation training was attenuated by ZIP.

Conclusion: We discuss this enriched rehabilitation training adaptive response to stroke as a potential underlying mechanism, which may play a role in improving synaptic plasticity and cognitive dysfunction.

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强化康复训练可改善慢性脑灌注不足大鼠的认知功能障碍

目的:探讨强化康复训练改善缺血性脑卒中认知功能障碍的分子机制。材料与方法:建立脑缺血合并认知功能障碍大鼠模型，按不同治疗方法分为4组。各组均进行水迷宫训练和认知功能评估。采用Western blot和免疫荧光法分析海马突触可塑性相关蛋白、调节蛋白和PKMζ的表达。采用膜片钳技术记录海马区场电位变化，分析LTP的诱导作用，并观察加入PKMζ抑制剂后场电位和LTP的变化。结果:强化康复训练可改善脑卒中大鼠的认知功能，提高缺血海马PKMζ和SYN蛋白水平，增强LTP。强化康复训练后大鼠LTP的增强被ZIP所减弱。结论:我们认为这种强化康复训练对脑卒中的适应性反应可能在改善突触可塑性和认知功能障碍中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroscience informatics Surgery, Radiology and Imaging, Information Systems, Neurology, Artificial Intelligence, Computer Science Applications, Signal Processing, Critical Care and Intensive Care Medicine, Health Informatics, Clinical Neurology, Pathology and Medical Technology

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57 days