Konvencionalna i funkcionalna procjena sjemena mužjaka mliječnih koza

Q3 Veterinary

Veterinarska stanica Pub Date : 2022-09-01 DOI:10.46419/vs.54.2.1

Daniela Herrera Vargas, Maricela Castaño Escobar, Henry Cardona Cadavid, Walter D. Cardona-Maya, Jose Manuel Mayorga-Torres, Jorge López Pérez, Daniela Arcila Davila

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引用次数: 0

Abstract

Evaluating the reproductive indicators in sheep breeding in Colombia, particularly males, is essential to achieve optimal production parameters. This study aimed to evaluate the semen quality of male goats through both conventional and functional seminal analysis. Semen samples from eight goats were collected and conventional (volume, appearance, colour, motility, vigour, and morphology) and functional (plasma membrane integrity, mitochondrial membrane potential, DNA integrity, lipid peroxidation, and reactive oxygen species production) seminal parameters were evaluated. The results showed an average scrotal circumference of 25.69 cm, seminal volume of 0.51 mL, and concentration of 1936 x 106 sperm/mL. The average individual motility was 63.37%, vigour was four, and the percentage of abnormalities was 7.75%. Individual motility was correlated with the mitochondrial membrane potential (r=0.840, P=0.009) and reactive oxygen species production (r=-0.91, P=0.001). The average of cells with high mitochondrial membrane potential was 52.94%, while 39.29% were necrotic cells, the DNA fragmentation index average was 12.5%, reactive oxygen species production was 38.68%, lipoperoxidation analysis was 7.33% on average, and the integrity of the membrane was 54.77%. The results establish the parameters for the semen from Antioquia goats and confirm that goat semen is of good quality and could be used during artificial insemination programs.

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公奶山羊的常规及功能评价

评估哥伦比亚绵羊养殖的繁殖指标，特别是雄性绵羊，对于实现最佳生产参数至关重要。本研究旨在通过常规精液分析和功能性精液分析来评价雄山羊的精液质量。采集了8只山羊的精液样本，并评估了常规（体积、外观、颜色、运动性、活力和形态）和功能（质膜完整性、线粒体膜电位、DNA完整性、脂质过氧化和活性氧产生）精液参数。结果显示，平均阴囊周长为25.69厘米，精液体积为0.51毫升，精子浓度为1936 x 106个/mL。平均个体活力为63.37%，活力为4，异常率为7.75%。个体活力与线粒体膜电位（r=0.840，P=0.009）和活性氧产生（r=-0.91，P=0.001）相关。线粒体膜电位高的细胞平均为52.94%，而坏死细胞为39.29%，DNA断裂指数平均为12.5%，活性氧产生率为38.68%，脂质过氧化物分析平均为7.33%，膜完整性为54.77%。

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来源期刊

Veterinarska stanica Veterinary-Veterinary (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The goal of the journal is to provide an international platform for the publication of articles in the fields of veterinary and animal sciences, and biotechnology. The content of the journal is particularly dedicated to veterinary practitioners, but also to veterinary scientists and university professors, to encourage them to share their knowledge and experience on this platform. Manuscripts submitted to the journal may include: original scientific papers, review articles, short communications, professional articles, case reports, conference reports and literary records and reviews of new book either in Croatian or English languages.