The Synergistic Effect of Theranekron and Cisplatin on the Neuroblastoma (SH-SY5Y) Cell Line.

Turkish neurosurgery Pub Date : 2023-01-01 DOI:10.5137/1019-5149.JTN.43431-23.4

Numan Taspinar

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Abstract

Aim: To assess the combined antitumor potential of theranekron (Trn) and cisplatin in neuroblastoma (NB), a pediatric tumor characterized by high prevalence and mortality rates. The investigation involved examining the interaction between theranekron (10-100 ?M) and cisplatin (40 ?M) within this cell line.

Material and methods: The cytotoxicity of Trn was evaluated using MTT assays, cell volume analyses, apoptosis assessments, and measurements of mitochondrial membrane potential. These evaluations were conducted over a 24-hour incubation period.

Results: The coadministration of 100 µM Trn with cisplatin exhibited a cytotoxic effect of approximately 60%. Furthermore, it led to a reduction of up to 38% in cell volume. Notably, SH-SY5Y cells demonstrated an early apoptosis rate of 34.4%, accompanied by an eightfold decrease in mitochondrial membrane potential compared to the control group.

Conclusion: Trn demonstrated synergism and significantly enhanced the efficacy of cisplatin (p < 0.001). Nevertheless, further in-depth investigations are necessary to elucidate the intricacies of these synergistic effects.

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Theranekron和顺铂对神经母细胞瘤（SH-SY5Y）细胞系的协同作用。

目的：神经母细胞瘤（NB）是一种发病率和死亡率较高的儿童肿瘤，是本研究的重点。本研究旨在评估theranekron（Trn）和顺铂在该细胞系中的联合抗肿瘤潜力。研究了theranekron（10-100µM）和顺铂（40µM）之间的相互作用。材料和方法：使用MTT法、细胞体积分析、细胞凋亡评估和线粒体膜电位测量来评估theranekron的细胞毒性。这些评估是在24小时的潜伏期内进行的。结果：100µM theranekron与顺铂联合用药显示出约60%的细胞毒性作用。此外，它导致细胞体积减少了38%。值得注意的是，SH-SY5Y细胞表现出34.4%的早期凋亡率，与对照组相比，线粒体膜电位降低了八倍。结论：Theranekron显示出协同作用，并显著增强了顺铂的疗效（P 0.001）。然而，有必要进一步深入研究这些协同作用的复杂性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Turkish neurosurgery

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