The Synergistic Effect of Theranekron and Cisplatin on the Neuroblastoma (SH-SY5Y) Cell Line.

Numan Taspinar
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Abstract

Aim: To assess the combined antitumor potential of theranekron (Trn) and cisplatin in neuroblastoma (NB), a pediatric tumor characterized by high prevalence and mortality rates. The investigation involved examining the interaction between theranekron (10-100 ?M) and cisplatin (40 ?M) within this cell line.

Material and methods: The cytotoxicity of Trn was evaluated using MTT assays, cell volume analyses, apoptosis assessments, and measurements of mitochondrial membrane potential. These evaluations were conducted over a 24-hour incubation period.

Results: The coadministration of 100 µM Trn with cisplatin exhibited a cytotoxic effect of approximately 60%. Furthermore, it led to a reduction of up to 38% in cell volume. Notably, SH-SY5Y cells demonstrated an early apoptosis rate of 34.4%, accompanied by an eightfold decrease in mitochondrial membrane potential compared to the control group.

Conclusion: Trn demonstrated synergism and significantly enhanced the efficacy of cisplatin (p < 0.001). Nevertheless, further in-depth investigations are necessary to elucidate the intricacies of these synergistic effects.

Theranekron和顺铂对神经母细胞瘤(SH-SY5Y)细胞系的协同作用。
目的:神经母细胞瘤(NB)是一种发病率和死亡率较高的儿童肿瘤,是本研究的重点。本研究旨在评估theranekron(Trn)和顺铂在该细胞系中的联合抗肿瘤潜力。研究了theranekron(10-100µM)和顺铂(40µM)之间的相互作用。材料和方法:使用MTT法、细胞体积分析、细胞凋亡评估和线粒体膜电位测量来评估theranekron的细胞毒性。这些评估是在24小时的潜伏期内进行的。结果:100µM theranekron与顺铂联合用药显示出约60%的细胞毒性作用。此外,它导致细胞体积减少了38%。值得注意的是,SH-SY5Y细胞表现出34.4%的早期凋亡率,与对照组相比,线粒体膜电位降低了八倍。结论:Theranekron显示出协同作用,并显著增强了顺铂的疗效(P 0.001)。然而,有必要进一步深入研究这些协同作用的复杂性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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