Long-term cryopreservation of whole gingival tissue.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-01 Epub Date: 2023-10-18 DOI:10.1007/s10561-023-10115-y

Dipika Rakh, Anita Kuloli, Avinash Kharat, Avinash Sanap, Supriya Kheur, Ramesh Bhonde, D Gopalakrishnan

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Abstract

Stem cells obtained from the body tissue, such as adipose tissue, dental pulp and gingival tissue. Fresh tissue is often used to isolate and culture for regenerative medicine. However, availability of tissue as and when required is one of the measure issue in regenerative medicine. Cryopreservation of tissue provides benefit over tissue availability, storage for significant amount of period and helps preserve the original cell structures. The effects of cryopreservation of gingival tissue for mesenchymal stem cell (MSC) are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation on the long term survival the whole gingival biopsy tissue. We studied cell outgrowth, cell morphology, MSC surface-markers and differentiation of mesenchymal stem cells derived from cryopreserved gingiva. In this study, gingival tissue was cryopreserved for 3, 6, 9 months. Cryopreserved tissue has been thawed and cells were isolated by using explant culture method. The fresh and cryopreserved gingival tissue cells were cultured and characterized for surface marker analysis, CFU-f, population doubling time, and osteogenic, chondrogenic and adipogenic differentiation. The fresh and cryopreserved tissue has similar stem cell properties. Results indicate that cryopreservation of the entire gingival tissue does not affect the properties of stem cells. This opens door for gingival tissue banking for future use in periodontology and regenerative medicine.

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全牙龈组织的长期冷冻保存。

从身体组织中获得的干细胞，如脂肪组织、牙髓和牙龈组织。新鲜组织通常用于再生医学的分离和培养。然而，组织在需要时的可用性是再生医学中的一个衡量问题。组织的冷冻保存优于组织的可用性，可储存相当长的时间，并有助于保存原始细胞结构。牙龈组织冷冻保存对间充质干细胞（MSC）的影响尚未得到充分的证明；然而，这一过程对再生疗法具有越来越重要的意义。本研究检测了冷冻保存对整个牙龈活检组织长期存活的影响。我们研究了冷冻保存牙龈中间充质干细胞的细胞生长、细胞形态、MSC表面标志物和分化。在本研究中，牙龈组织被冷冻保存了3、6、9个月。冷冻保存的组织已经解冻，并使用外植体培养方法分离细胞。培养新鲜和冷冻保存的牙龈组织细胞，并对其进行表面标记物分析、CFU-f、群体倍增时间以及成骨、软骨和脂肪分化的表征。新鲜和冷冻保存的组织具有相似的干细胞特性。结果表明，冷冻保存整个牙龈组织不会影响干细胞的特性。这为牙龈组织库在牙周病学和再生医学中的未来应用打开了大门。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.