Urinary IL-6 and IL-8 as predictive markers in bladder urothelial carcinoma: A pilot study

IF 2.6 3区医学 Q3 ONCOLOGY

Cancer Cytopathology Pub Date : 2023-10-09 DOI:10.1002/cncy.22767

Christopher J. VandenBussche MD, PhD, Christopher D. Heaney PhD, MS, Max Kates MD, John J. Hooks PhD, Kelly Baloga MLS(ASCP)CM, Lori Sokoll PhD, Dorothy Rosenthal MD, Barbara Detrick PhD

{"title":"Urinary IL-6 and IL-8 as predictive markers in bladder urothelial carcinoma: A pilot study","authors":"Christopher J. VandenBussche MD, PhD, Christopher D. Heaney PhD, MS, Max Kates MD, John J. Hooks PhD, Kelly Baloga MLS(ASCP)CM, Lori Sokoll PhD, Dorothy Rosenthal MD, Barbara Detrick PhD","doi":"10.1002/cncy.22767","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Cytokines are known to be a key a factor in numerous malignancies and to exert an important regulatory role in the tumor microenvironment. Interest has grown in understanding how cytokines modulate the tumor microenvironment and which cytokines may serve as markers of the tumor process; however, a complete picture of the cytokine landscape in bladder cancer remains unclear.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Fresh urine specimens with sufficient volume were collected at random intervals. The urine concentrations of IL-8 (CXCL8), CCL18, and CXCL9 were determined using the standard commercially available enzyme immunoassay. The urine concentrations of IL-6 were determined using the high sensitivity enzyme immunoassay kit. Urinary cytokine concentrations were normalized with urinary creatinine concentrations.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Significantly elevated concentrations of IL-6 and IL-8 were detected in the urine from patients with urothelial carcinoma on follow-up compared to patients with benign follow-up. The presence of both IL-6 and IL-8 in the urine samples from the high grade urothelial carcinoma (HGUC) cohort revealed a clear discrimination when compared to samples from patients with benign follow-up. The presence of the combination of both IL-6 and IL-8 had a sensitivity of 90.0% and a specificity of 81.25%. Similar data were obtained when receiver operating characteristic analysis was performed on both IL-6 and IL-8 concentrations in the urine from patients with HGUC vs. the hematuria cohort.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The presence of IL-6 and IL-8 in urine specimens may have predictive value for urothelial carcinoma. However, a large longitudinal study is required to statistically eliminate confounding factors and support this theory.</p>\n </section>\n </div>","PeriodicalId":9410,"journal":{"name":"Cancer Cytopathology","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer Cytopathology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cncy.22767","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Cytokines are known to be a key a factor in numerous malignancies and to exert an important regulatory role in the tumor microenvironment. Interest has grown in understanding how cytokines modulate the tumor microenvironment and which cytokines may serve as markers of the tumor process; however, a complete picture of the cytokine landscape in bladder cancer remains unclear.

Methods

Fresh urine specimens with sufficient volume were collected at random intervals. The urine concentrations of IL-8 (CXCL8), CCL18, and CXCL9 were determined using the standard commercially available enzyme immunoassay. The urine concentrations of IL-6 were determined using the high sensitivity enzyme immunoassay kit. Urinary cytokine concentrations were normalized with urinary creatinine concentrations.

Results

Significantly elevated concentrations of IL-6 and IL-8 were detected in the urine from patients with urothelial carcinoma on follow-up compared to patients with benign follow-up. The presence of both IL-6 and IL-8 in the urine samples from the high grade urothelial carcinoma (HGUC) cohort revealed a clear discrimination when compared to samples from patients with benign follow-up. The presence of the combination of both IL-6 and IL-8 had a sensitivity of 90.0% and a specificity of 81.25%. Similar data were obtained when receiver operating characteristic analysis was performed on both IL-6 and IL-8 concentrations in the urine from patients with HGUC vs. the hematuria cohort.

Conclusions

The presence of IL-6 and IL-8 in urine specimens may have predictive value for urothelial carcinoma. However, a large longitudinal study is required to statistically eliminate confounding factors and support this theory.

查看原文本刊更多论文

尿IL-6和IL-8作为膀胱尿路上皮癌的预测标志物：一项初步研究。

背景：众所周知，细胞因子是许多恶性肿瘤的关键因子，并在肿瘤微环境中发挥重要的调节作用。人们对了解细胞因子如何调节肿瘤微环境以及哪些细胞因子可以作为肿瘤过程的标志物越来越感兴趣；然而，细胞因子在膀胱癌症中的完整情况仍不清楚。方法：随机采集足够容量的新鲜尿液标本。使用标准市售酶免疫测定法测定IL-8（CXCL8）、CCL18和CXCL9的尿液浓度。使用高灵敏度酶免疫测定试剂盒测定尿液中IL-6的浓度。尿细胞因子浓度用尿肌酐浓度标准化。结果：与良性随访患者相比，随访中尿路上皮癌患者尿液中IL-6和IL-8浓度显著升高。与良性随访患者的尿液样本相比，来自高级别尿路上皮癌（HGUC）队列的尿液样本中IL-6和IL-8的存在显示出明显的区别。IL-6和IL-8联合存在的敏感性为90.0%，特异性为81.25%。当对HGUC患者与血尿队列的尿液中IL-6和IL-8%浓度进行受试者操作特征分析时，获得了类似的数据。结论：尿液中IL-6和IL-8的存在可能对尿路上皮癌具有预测价值。然而，需要进行大规模的纵向研究，以从统计上消除混杂因素并支持这一理论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cancer Cytopathology 医学-病理学

CiteScore

7.00

自引率

17.60%

发文量

130

审稿时长

1 months

期刊介绍： Cancer Cytopathology provides a unique forum for interaction and dissemination of original research and educational information relevant to the practice of cytopathology and its related oncologic disciplines. The journal strives to have a positive effect on cancer prevention, early detection, diagnosis, and cure by the publication of high-quality content. The mission of Cancer Cytopathology is to present and inform readers of new applications, technological advances, cutting-edge research, novel applications of molecular techniques, and relevant review articles related to cytopathology.