Aslihan Sur, Seda Iflazoglu Mutlu, Pinar Tatli Seven, Ismail Seven, Abdullah Aslan, Meltem Kizil, Recai Kulaksiz, Mustafa Hilmi Yaranoglu, Selim Esen
{"title":"Effects of grape seed proanthocyanidin extract on side effects of high-dose methylprednisolone administration in male rats.","authors":"Aslihan Sur, Seda Iflazoglu Mutlu, Pinar Tatli Seven, Ismail Seven, Abdullah Aslan, Meltem Kizil, Recai Kulaksiz, Mustafa Hilmi Yaranoglu, Selim Esen","doi":"10.1007/s43188-023-00196-y","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, we investigated the effects of grape seed proanthocyanidin extract (GSPE) against the side effects of high-dose administration of methylprednisolone (MP) in male rats. A total of 32 adult Wistar male albino rats were divided into four groups: (1) control (CON), received standard food only; (2) MP, received standard food + intraperitoneal injection of 60 mg/kg MP on day 7; (3) GSPE, received standard food + 200 mg/kg/day GSPE; and (4) MP + GSPE, received standard food + 200 mg/kg/day of GSPE + intraperitoneal injection of 60 mg/kg MP on day 7. All animals in the GSPE and GSPE + MP groups were treated once a day by oral gavage for 14 consecutive days. The feed intake of rats in the MP and MP + GSPE groups decreased significantly by 24.14% and 13.52%, respectively (<i>p</i> < 0.05). Administration of MP resulted in significant increases in serum concentrations of blood urea nitrogen (<i>p</i> < 0.001), glucose (<i>p</i> < 0.01), alkaline phosphatase, and adrenocorticotropic hormone (<i>p</i> < 0.05). High-dose MP administration significantly reduced catalase (<i>p</i> < 0.001) and glutathione peroxidase (<i>p</i> < 0.05) concentrations in the liver and kidney tissues of rats, while glutathione concentrations were only reduced in liver tissue (<i>p</i> < 0.05). The expression levels of <i>Bcl-2</i> and <i>TNF-α</i> in liver, kidney, and testicular tissue were significantly increased, while the expression levels of <i>caspase-3</i> were reduced (<i>p</i> < 0.001). Furthermore, sperm concentration was significantly affected by GSPE in rats induced by high-dose MP, and sperm loss was significantly reduced in MP + GSPE (<i>p</i> < 0.05). These findings suggest that GSPE could be useful as a supplement to alleviate MP-induced toxicity in rats.</p>","PeriodicalId":23181,"journal":{"name":"Toxicological Research","volume":"39 4","pages":"749-759"},"PeriodicalIF":1.6000,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541365/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s43188-023-00196-y","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/10/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, we investigated the effects of grape seed proanthocyanidin extract (GSPE) against the side effects of high-dose administration of methylprednisolone (MP) in male rats. A total of 32 adult Wistar male albino rats were divided into four groups: (1) control (CON), received standard food only; (2) MP, received standard food + intraperitoneal injection of 60 mg/kg MP on day 7; (3) GSPE, received standard food + 200 mg/kg/day GSPE; and (4) MP + GSPE, received standard food + 200 mg/kg/day of GSPE + intraperitoneal injection of 60 mg/kg MP on day 7. All animals in the GSPE and GSPE + MP groups were treated once a day by oral gavage for 14 consecutive days. The feed intake of rats in the MP and MP + GSPE groups decreased significantly by 24.14% and 13.52%, respectively (p < 0.05). Administration of MP resulted in significant increases in serum concentrations of blood urea nitrogen (p < 0.001), glucose (p < 0.01), alkaline phosphatase, and adrenocorticotropic hormone (p < 0.05). High-dose MP administration significantly reduced catalase (p < 0.001) and glutathione peroxidase (p < 0.05) concentrations in the liver and kidney tissues of rats, while glutathione concentrations were only reduced in liver tissue (p < 0.05). The expression levels of Bcl-2 and TNF-α in liver, kidney, and testicular tissue were significantly increased, while the expression levels of caspase-3 were reduced (p < 0.001). Furthermore, sperm concentration was significantly affected by GSPE in rats induced by high-dose MP, and sperm loss was significantly reduced in MP + GSPE (p < 0.05). These findings suggest that GSPE could be useful as a supplement to alleviate MP-induced toxicity in rats.
在本研究中,我们研究了葡萄籽原花青素提取物(GSPE)对雄性大鼠大剂量服用甲基强的松龙(MP)副作用的影响。将32只成年Wistar雄性白化大鼠分为四组:(1)对照组(CON),仅接受标准食品;(2) MP,接受标准食品 + 在第7天腹膜内注射60mg/kg MP;(3) GSPE,收到标准食品 + 200mg/kg/天的GSPE;和(4)MP + GSPE,收到标准食品 + 200mg/kg/天的GSPE + 在第7天腹膜内注射60mg/kg MP。GSPE和GSPE中的所有动物 + MP组每天口服一次,连续14天。MP和MP中大鼠的采食量 + GSPE组分别下降24.14%和13.52%(p p p p p p p Bcl-2和TNF-α在肝、肾和睾丸组织中的表达显著增加,而胱天蛋白酶-3的表达水平降低(p p
期刊介绍:
Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.