Understanding exosomes: Part 1-Characterization, quantification and isolation techniques.

IF 17.5 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Periodontology 2000 Pub Date : 2024-02-01 Epub Date: 2023-09-22 DOI:10.1111/prd.12520
Richard J Miron, Yufeng Zhang
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引用次数: 0

Abstract

Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with a diameter in the range of 30-150 nm. Their use has gained great momentum recently due to their ability to be utilized as diagnostic tools with a vast array of therapeutic applications. Over 5000 publications are currently being published yearly on this topic, and this number is only expected to dramatically increase as novel therapeutic strategies continue to be investigated. This review article first focuses on understanding exosomes, including their cellular origin, biogenesis, function, and characterization. Thereafter, overviews of the quantification methods and isolation techniques are given with discussion over their potential use as novel therapeutics in regenerative medicine.

理解外泌体:第1部分——表征、定量和分离技术。
外泌体是大多数细胞分泌的细胞外信号小泡的最小子集,直径在30-150nm范围内。由于它们能够被用作具有广泛治疗应用的诊断工具,因此它们的使用最近获得了巨大的势头。目前每年出版5000多篇关于这一主题的出版物,随着新的治疗策略的不断研究,这一数字预计只会急剧增加。这篇综述文章首先集中于理解外泌体,包括它们的细胞起源、生物发生、功能和表征。随后,对定量方法和分离技术进行了综述,并讨论了它们在再生医学中作为新疗法的潜在用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Periodontology 2000
Periodontology 2000 医学-牙科与口腔外科
CiteScore
34.10
自引率
2.20%
发文量
62
审稿时长
>12 weeks
期刊介绍: Periodontology 2000 is a series of monographs designed for periodontists and general practitioners interested in periodontics. The editorial board selects significant topics and distinguished scientists and clinicians for each monograph. Serving as a valuable supplement to existing periodontal journals, three monographs are published annually, contributing specialized insights to the field.
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