tRNA-Uridine Aminocarboxypropyltransferase DTW Domain Containing 2 Suppresses Colon Adenocarcinoma Progression.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Genomics Pub Date : 2023-09-16 eCollection Date: 2023-01-01 DOI:10.1155/2023/4354536

Yun Qian, Yu-Jiang Li, Yi-Wei Fu, Cui-Xia Liu, Juan Wang, Bin Yang

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引用次数: 0

Abstract

Background: DTW Domain Containing 2 (DTWD2) is a newly identified transfer RNA-uridine aminocarboxypropyltransferase. Dysregulated expression of DTWD1 has been reported in several malignancies, nevertheless, the role of DTWD2 in cancers remains completely unknown. Here, we aimed to initially investigate the expression and role of DTWD2 in colon adenocarcinoma.

Methods: We first evaluated the transcription and mRNA levels of DTWD2 using data from The Cancer Genome Atlas. Besides, we tested its mRNA and protein expression in our enrolled retrospective cohort. Univariate and multivariate analyses were conducted to assess its prognostic value. Cellular experiments and xenografts were also performed to validate the role of DTWD2 in colon cancer progression.

Results: DTWD2 was downregulated in colon adenocarcinoma and associated with poor prognosis. Lymph node metastasis, distant metastasis, and advanced tumor stage are all characterized by lower DTWD2 levels. Furthermore, Cox regression analysis demonstrated that DTWD2 is a novel independent prognostic factor for colon cancer patients. Finally, cellular and xenograft data demonstrated that silencing DTWD2 significantly enhanced colon cancer growth.

Conclusion: Low expression of DTWD2 may be a potential molecular marker for poor prognosis in colon cancer.

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tRNA尿苷氨基羧丙基转移酶DTW结构域含2抑制结肠腺癌的进展。

背景：DTW结构域含2（DTWD2）是一种新发现的转移核糖核酸尿苷氨基羧丙基转移酶。据报道，DTWD1在几种恶性肿瘤中表达失调，然而，DTWD2在癌症中的作用仍然完全未知。在此，我们旨在初步研究DTWD2在结肠腺癌中的表达和作用。方法：我们首先使用癌症基因组图谱的数据评估了DTWD2的转录和mRNA水平。此外，我们在纳入的回顾性队列中测试了其mRNA和蛋白质表达。进行单变量和多变量分析以评估其预后价值。还进行了细胞实验和异种移植物以验证DTWD2在结肠癌癌症进展中的作用。结果：DTWD2在结肠癌中表达下调，预后不良。淋巴结转移、远处转移和晚期肿瘤都以DTWD2水平较低为特征。此外，Cox回归分析表明，DTWD2是结肠癌癌症患者的一个新的独立预后因素。最后，细胞和异种移植物数据表明，沉默DTWD2显著增强了癌症的生长。结论：DTWD2低表达可能是癌症预后不良的潜在分子标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Genomics BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： International Journal of Genomics is a peer-reviewed, Open Access journal that publishes research articles as well as review articles in all areas of genome-scale analysis. Topics covered by the journal include, but are not limited to: bioinformatics, clinical genomics, disease genomics, epigenomics, evolutionary genomics, functional genomics, genome engineering, and synthetic genomics.