Neuroprotective Effect of Ethanolic Extract of Scoparia dulcis on Acrylamide-Induced Neurotoxicity in Zebrafish Model

IF 3.1 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Applied Biochemistry and Biotechnology Pub Date : 2023-10-06 DOI:10.1007/s12010-023-04733-1

Trisha Raghunathan, Subhiksha Srinivasan, Sankar Jamuna

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Abstract

All herbal medicines are reported to be safe and have better results in curing disabilities. Scoparia dulcis is known for its anti-inflammatory and antioxidant properties. This study has been executed to explore the neuroprotective effects of ethanolic extract of Scoparia dulcis (EESD) against acrylamide using adult zebrafish. The experimental period was 72 h. After fixing the optimum acrylamide concentration and EESD, the healthy adult fish were grouped into control, induction, and treatment. During the experimental period, behavioural changes such as memory and locomotion were observed in control and experimental groups using the T-maze experiment. After 72 h, the neuronal tissues were isolated from the grouped fishes and analysed for various biochemical and enzymatic assays. The mRNA of the HSP-70 gene in control and experimental groups was expressed using RT-PCR. The optimum dosages for acrylamide and EESD were found to be 0.75 mM and 20 µg/mL, respectively. Memory improvement was observed in S. dulcis-treated fish, compared to the acrylamide-treated group using the T-maze assay. The extract reduced the toxicity induced by acrylamide from the various biochemical and histopathological parameters. The result shows the potential neuroprotective effects of ethanolic extract of Scoparia dulcis (EESD) against acrylamide-induced neurotoxicity in adult zebrafish. Therefore, Scoparia dulcis is a potent neuroprotective agent.

Graphical Abstract

Abstract Image

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Scoparia dulcis乙醇提取物对斑马鱼模型中丙烯酰胺诱导的神经毒性的神经保护作用。

据报道，所有草药都是安全的，在治疗残疾方面效果更好。Scoparia dulcis以其抗炎和抗氧化特性而闻名。本研究旨在探讨Scoparia dulcis乙醇提取物（EESD）对成年斑马鱼丙烯酰胺的神经保护作用。实验时间为72小时。确定最佳丙烯酰胺浓度和EESD后，将健康成鱼分为对照组、诱导组和处理组。在实验期间，使用T迷宫实验，在对照组和实验组中观察到行为变化，如记忆和运动。72小时后，从分组的鱼类中分离神经元组织，并进行各种生化和酶测定分析。对照组和实验组HSP-70基因的mRNA用RT-PCR表达。丙烯酰胺和EESD的最佳剂量分别为0.75 mM和20µg/mL。使用T迷宫测定法，与丙烯酰胺处理组相比，杜尔西处理组的鱼记忆力得到改善。从各种生化和组织病理学参数来看，该提取物降低了丙烯酰胺诱导的毒性。结果表明，Scoparia dulcis乙醇提取物（EESD）对丙烯酰胺诱导的成年斑马鱼神经毒性具有潜在的神经保护作用。因此，Scoparia dulcis是一种有效的神经保护剂。

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来源期刊

Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学

CiteScore

5.70

自引率

6.70%

发文量

460

审稿时长

5.3 months

期刊介绍： This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.