Homeotic transformation in a terrestrial isopod: insights into the appendage identity in crustaceans

Abstract

In many crustacean species, an individual possesses both uniramous and biramous appendages that enable us to compare the two types on the same genetic background. Therefore, among the diverse morphologies of arthropod appendages, crustacean biramous appendages provide interesting subjects for studying the developmental mechanisms underlying appendage modifications. In this study, we report a malformed specimen of the terrestrial isopod Porcellio scaber, in which one of the pleopods was transformed into a different structure. Morphological observations of exoskeletons and musculatures by confocal scanning laser microscopy revealed that the transformed appendage was three-segmented, with at least the apical two segments having pereopod-like musculoskeletal structures. The apical segment of the transformed appendage lacked muscles, and the following segment had a pair of muscle bundles. These findings together with those of some previous studies of gene expression patterns in this species suggest that this anomaly could be caused by homeotic transformation of a flap-like pleopod into a three-segmented pereopod tip, which may be a homologous structure of the pleopod.