Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2023-10-06 DOI:10.1016/j.anaerobe.2023.102784

Yuriko Matsumiya , Mitsukuni Suenaga , Toshiaki Ishikawa , Toshifumi Kudo , Tsuyoshi Nakagawa , Kentaro Okamoto , Masanori Tokunaga , Claudia Hurtado , Yuki Yamada , Kentaro Oka , Motomichi Takahashi , Luis Francisco Lopez Kostner , Miguel Luis O'Ryan Gallardo , Hiroyuki Uetake , Yusuke Kinugasa

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引用次数: 0

Abstract

Introduction

Bacteroides fragilis (B. fragilis) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic B. fragilis (ETBF), a subtype of B. fragilis, produces an enterotoxin (BFT; B. fragilis toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of B. fragilis and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection.

Methods

We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of B. fragilis and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed.

Results

16 S rRNA for B. fragilis and bft DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. B. fragilis-positive patients had better RFS than B. fragilis-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of B. fragilis in pstage II and left-sided CRC. The association of B. fragilis positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic B. fragilis.

Conclusions

Our findings suggest that the presence of B. fragilis is associated with better outcomes in patients with pstage II and III CRC after curative resection.

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脆弱拟杆菌作为癌症潜在预后因素的临床意义。

简介：脆弱拟杆菌被认为对肠道具有抗炎作用。相反，产肠毒素的脆弱双歧杆菌（ETBF），脆弱双歧杆菌的一种亚型，产生肠毒素（BFT；脆弱双歧杆菌毒素），导致无症状的慢性感染和结肠肿瘤形成。然而，脆弱B.fragilis和ETBF对癌症（CRC）临床结果的影响仍不清楚。我们的目的是评估它们的存在是否会影响CRC患者根治性切除后的结果。方法：我们从病理分期（pstage）II和III期CRC患者的癌组织和邻近非癌组织中获得197对匹配的福尔马林固定石蜡包埋样品。B。使用实时聚合酶链反应评估脆弱性和ETBF，并分析患者的无复发生存率（RFS）和总生存率（OS）。结果：在197例患者中，分别检测到120例（60.9%）和12例（6.1%）脆弱双歧杆菌16S rRNA和bft DNA。脆弱双歧杆菌阳性患者的RFS优于脆弱双歧杆菌阴性患者，尽管这在统计学上并不显著。在亚组分析中，在pstage II和左侧CRC中存在脆弱双歧杆菌的情况下，观察到RFS的更好结果。脆弱双歧杆菌阳性与OS的相关性在T4亚组的深度中更为突出。ETBF和无毒脆弱B.fragilis之间的RFS和OS没有观察到显著差异。结论：我们的研究结果表明，脆弱双歧杆菌的存在与II期和III期CRC患者在根治性切除后的更好结果有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.