Host-virus interaction between tobacco mild green mosaic virus strain U2 and tropical soda apple resulting in systemic hypersensitive necrosis and the host range, survival, spread, and molecular characterization of the virus

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2023-09-28 DOI:10.1002/ps.7802

Raghavan Charudattan, Ernest Hiebert, Matthew S. Pettersen, Jonathan R. Horrell, Mark S. Elliott, James T. DeValerio, Gabriella S. Maia, Thais Balbao Clemente Bueno de Oliveira

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Abstract

BACKGROUND

Tobacco mild green mosaic virus strain U2 (TMGMV-U2) is a registered active ingredient in a bioherbicide to control tropical soda apple (TSA), Solanum viarum, an invasive weed. As required for registration, we developed empirical data on the host-virus interaction and the virus's host range, survival, spread, and genomic sequence.

RESULTS

TMGMV-U2 killed TSA plants by causing systemic hypersensitive necrosis (SHN). It elicited local lesions in inoculated leaves which was followed by the plant's wilting and death. It moved from inoculated terminal leaves through the vasculature to roots and then to newly developed leaves. Phloem death was implicated in wilting and plant death. The SHN response was attenuated in plants grown at constant 32 °C. TMGMV-U2 titer in TSA was low compared to a systemically susceptible tobacco. The virus remained infective for up to 6 months in infected dead TSA tissues and in soil in which infected plants had grown. Susceptible tobacco and pepper plants grown in soil that previously had infected dead TSA or in soil amended with the virus remained asymptomatic and virus-free. A susceptible pepper crop grown in a field block following two consecutive crops of TMGMV-U2-infected susceptible tobacco grew disease-free and virus-free and without yield loss. Purified TMGMV-U2 was infective for 1 year when stored at −20 °C or 5 °C and for 1 month at room temperature. No virus spread was found in the field. Genomic analyses confirmed the registered isolate to be a U2 strain and free of satellite TMV. The TMGMV-U2-susceptible species preponderantly belonged to the Solanaceae. A few hosts that were killed belonged to this family. Several new hosts to TMGMV-U2 were found. These data enabled registration of TMGMV-U2.

CONCLUSION

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烟草温和绿色花叶病毒U2株与热带苏打苹果之间的宿主病毒相互作用导致系统性超敏坏死，以及病毒的宿主范围、存活、传播和分子特征。

背景：烟草温和绿色花叶病毒U2株（TMGMV-U2）是一种生物除草剂中的注册活性成分，用于控制热带苏打苹果（TSA），即入侵杂草龙葵。根据注册要求，我们开发了关于宿主-病毒相互作用和病毒宿主范围、存活、传播和基因组序列的经验数据。结果：TMGMV-U2通过引起全身性超敏性坏死（SHN）杀死TSA植物。它在接种的叶片中引起局部损伤，随后植物枯萎死亡。它从接种的末端叶片通过脉管系统移动到根部，然后移动到新发育的叶片。Phloem的死亡与枯萎和植物的死亡有关。在恒定32°C下生长的植物中，SHN反应减弱。TSA中TMGMV-U2滴度与系统敏感烟草相比较低。该病毒在受感染的死亡TSA组织和生长受感染植物的土壤中保持感染长达6个月。生长在先前感染过死亡TSA的土壤中或经过病毒改良的土壤中的易感烟草和辣椒植物仍然没有症状且无病毒。在连续两次种植TMGMV-U2感染的易感烟草后，在田间种植的易感辣椒作物生长无病、无病毒，且没有产量损失。纯化TMGMV-U2在-20°C或5°C下储存1年，在室温下储存1个月，具有感染性。现场没有发现病毒传播。基因组分析证实，登记的分离株是U2菌株，不含卫星TMV。TMGMV-U2-敏感种主要为茄科植物。一些被杀的宿主属于这个家族。TMGMV-U2发现了几个新的宿主。结论：TMGMV-U2可以安全地用作生物除草剂，对非靶标植物和环境没有风险。这篇文章受版权保护。保留所有权利。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.