Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal–Organic Frameworks-Based Immunobeads

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2023-09-22 DOI:10.1021/acs.nanolett.3c02828

Fengli Chai, Dou Wang, Fei Shi, Wenfu Zheng, Xiaohui Zhao, Yao Chen, Cuiping Mao, Jiangjiang Zhang* and Xingyu Jiang*,

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Abstract

Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (∼80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.

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使用基于金属有机框架的免疫珠的双功能超灵敏护理点临床诊断。

脓毒症是一种致死率很高的急性全身感染综合征。败血症的快速准确诊断、监测和药物治疗至关重要。我们利用了荧光金属AIEgen框架（MAFs），并证明了蛋白质检测和细菌识别的双重功能：（i）败血症生物标志物的超灵敏护理点（POC）检测（灵敏度提高100倍）；（ii）革兰氏阴性/阳性细菌的快速POC鉴定（20分钟内选择性聚集）。荧光侧流免疫测定法（LFA）对于POC测试是方便且廉价的。MAFs具有大的表面积、优异的光稳定性、高量子产率（~80%）和多个活性位点，可作为LFA上败血症生物标志物（IL-6/PCT）的超灵敏检测的蛋白质结合结构域。IL-6/PCT的检测限为0.252/0.333pg/mL。对传染性细菌的快速鉴定对于指导药物的使用至关重要。双功能荧光MAFs在POC测试中对细菌感染的临床诊断具有巨大潜力。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.